NC2 (Dr1-Drap1 or Bur6-Ydr1) has been characterized in vitro as a general negative regulator of RNA polymerase II (Pol II) transcription that interacts with TATA-binding protein (TBP) and inhibits its function. Here, we show that NC2 associates with promoters in vivo in a manner that correlates with transcriptional activity and with occupancy by basal transcription factors. NC2 rapidly associates with promoters in response to transcriptional activation, and it remains associated under conditions in which transcription is blocked after assembly of the Pol II preinitiation complex. NC2 positively and negatively affects approximately 17% of Saccharomyces cerevisiae genes in a pattern that resembles the response to general environmental stress. Relative to TBP, NC2 occupancy is high at promoters where NC2 is positively required for normal levels of transcription. Thus, NC2 is associated with the Pol II preinitiation complex, and it can play a direct and positive role at certain promoters in vivo.
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| Gene/Complex | Qualifier | Gene Ontology Term | Annotation Extension | Evidence | Source | Assigned On |
|---|---|---|---|---|---|---|
| Negative cofactor 2 transcriptional regulator complex | located in | nucleus | BSR | ComplexPortal | 2014-10-16 | |
| Negative cofactor 2 transcriptional regulator complex | enables | RNA polymerase II core promoter sequence-specific DNA binding | IPI | ComplexPortal | 2014-10-16 | |
| Negative cofactor 2 transcriptional regulator complex | involved in | negative regulation of RNA polymerase II transcription preinitiation complex assembly | IDA | ComplexPortal | 2014-10-16 |