The RNA polymerase II general transcription factor TFIID is a multisubunit complex comprising TATA-box binding protein and associated factors (TAFIIs). In vitro experiments have suggested that TAFIIs are essential coactivators required for RNA polymerase II-directed transcription activation. Here, for the first time, we analyze systematically the in vivo function of a specific TAFII, yeast TAFII90 (yTAFII90). We show that functional inactivation of yTAFII90 by temperature-sensitive mutations or depletion leads to arrest at the G2/M phase of the cell cycle. Unexpectedly, in the absence of functional yTAFII90, a variety of endogenous yeast genes were all transcribed normally, including those driven by well-characterized activators. Taken together, our results indicate that yTAFII90 is not required for transcription activation in general, and reveal linkages between TAF function and cell-cycle progression.
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| Gene | Phenotype | Experiment Type | Mutant Information | Strain Background | Chemical | Details |
|---|---|---|---|---|---|---|
| TAF5 | cell cycle progression through the G2/M phase transition: arrested | classical genetics | conditional Allele: taf5-(ytafII90ts3-1) G712E, G713S; WD40 domain mutated | S288C | Temperature: elevated temperature, 37 °C Details: large budded arrest (84% at 4 hours), with a single nucleus located near the bud neck and 2N DNA content | |
| TAF5 | cell cycle progression: arrested | classical genetics | repressible | S288C | Details: large budded arrest (74% at 12 hours) | |
| TAF5 | heat sensitivity: increased | classical genetics | conditional Allele: taf5-(ytafII90ts3-1) G712E, G713S; WD40 domain mutated | S288C | Temperature: elevated temperature, 37 °C |