Reference: Espinoza FH, et al. (1994) Cell cycle control by a complex of the cyclin HCS26 (PCL1) and the kinase PHO85. Science 266(5189):1388-91

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Abstract


The events of the eukaryotic cell cycle are governed by cyclin-dependent kinases (cdk's), whose activation requires association with cyclin regulatory subunits expressed at specific cell cycle stages. In the budding yeast Saccharomyces cerevisiae, the cell cycle is thought to be controlled by a single cdk, CDC28. Passage through the G1 phase of the cell cycle is regulated by complexes of CDC28 and G1 cyclins (CLN1, CLN2, and CLN3). A putative G1 cyclin, HCS26, has recently been identified. In a/alpha diploid cells lacking CLN1 and CLN2, HCS26 is required for passage through G1. HCS26 does not associate with CDC28, but instead associates with PHO85, a closely related protein kinase. Thus, budding yeast, like higher eukaryotes, use multiple cdk's in the regulation of cell cycle progression.

Reference Type
Journal Article | Research Support, Non-U.S. Gov't | Research Support, U.S. Gov't, P.H.S.
Authors
Espinoza FH, Ogas J, Herskowitz I, Morgan DO
Primary Lit For
CLN1 | PCL1 | PHO85 | PCL1-PHO85 kinase complex
Additional Lit For
CDC28 | CLN3 | CLN2 | CLB3-CDC28 kinase complex

Gene Ontology Annotations 4 entries for 2 genes


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Gene/ComplexQualifierGene Ontology TermAnnotation ExtensionEvidenceSourceAssigned On
PCL1part ofcyclin-dependent protein kinase holoenzyme complexIPI with PHO85SGD2013-08-07
PCL1enablescyclin-dependent protein serine/threonine kinase regulator activityhappens during mitotic G1 phaseIDASGD2013-10-14
PHO85enablescyclin-dependent protein serine/threonine kinase activityIDASGD2013-08-07
PHO85part ofcyclin-dependent protein kinase holoenzyme complexIPI with PCL1SGD2013-08-07
Showing 1 to 4 of 4 entries

Interaction Annotations


Genetic Interactions

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Interactor Interactor Allele Assay Annotation Action Phenotype SGA score P-value Source Reference

Physical Interactions 3 entries for 5 genes

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InteractorInteractorAssayAnnotationActionModification
CDC28CLB3Affinity Capture-Westernmanually curatedHit-BaitNo Modification
PCL1PHO85Affinity Capture-Westernmanually curatedBait-HitNo Modification
PHO4PCL1Affinity Capture-Westernmanually curatedHit-BaitNo Modification
Showing 1 to 3 of 3 entries