The complete sequences of the yeast a mating-type locus, MATa, and of the silent alpha cassette, HML alpha, have been determined. A segment of 642 nucleotides is unique to MATa, and a corresponding segment of 747 nucleotides is unique to MAT alpha. The major mRNAs (a1, a2, alpha 1 and alpha 2) encoded by MATa and MAT alpha have been aligned with the DNA sequence. The a1 mRNA is encoded entirely within the a-specific DNA sequence. The a2 mRNA, which is transcribed divergently from a1 mRNA, is encoded in a region common to both Mata and Mat alpha. The alpha 1 and alpha 2 mRNAs are also transcribed divergently and have their 5' starts about 240 nucleotides apart within the alpha-specific sequence. The amino acid sequences of the MAT proteins have been predicted from the DNA sequences. An unanticipated conclusion is that the a1 protein, containing 148 amino acids, results from readthrough of a UGA at codon 45. Polymorphic forms of the homologous outer segments of the HML alpha, MAT alpha, MATa and HMRa sequences suggest that the boundaries of the segments involved in mating-type switching are immediately adjacent to the a-specific and alpha-specific sequences.
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| Gene/Complex | Qualifier | Gene Ontology Term | Annotation Extension | Evidence | Source | Assigned On |
|---|---|---|---|---|---|---|
| HMLALPHA1 | enables | DNA binding, bending | ISA with MATALPHA1 | SGD | 2013-08-07 | |
| HMLALPHA1 | located in | nucleus | ISA with MATALPHA1 | SGD | 2013-08-07 | |
| HMLALPHA1 | involved in | regulation of transcription by RNA polymerase II | ISA with MATALPHA1 | SGD | 2013-08-07 | |
| HMLALPHA1 | involved in | regulation of mating-type specific transcription, DNA-templated | ISA with MATALPHA1 | SGD | 2013-08-07 | |
| HMLALPHA1 | enables | transcription coactivator activity | ISA with MATALPHA1 | SGD | 2013-08-07 | |
| HMLALPHA2 | involved in | mating type determination | ISA with MATALPHA2 | SGD | 2022-08-11 | |
| HMLALPHA2 | enables | DNA binding, bending | ISA with MATALPHA2 | SGD | 2014-10-31 | |
| HMLALPHA2 | located in | nucleus | ISA with MATALPHA2 | SGD | 2013-08-07 | |
| HMLALPHA2 | enables | DNA-binding transcription repressor activity, RNA polymerase II-specific | ISA with MATALPHA2 | SGD | 2013-08-07 | |
| HMLALPHA2 | involved in | negative regulation of transcription by RNA polymerase II | part of mating type determination | ISA with MATALPHA2 | SGD | 2022-08-11 |