The purpose of this work is to characterize the structural difference of conjugates in order to condition the immunoreactivity of the enzymatic tracer in the homogeneous immunoassays. Conjugates between yeast alcohol dehydrogenase (ADH) and 7-theophyllincarboxyalkyl acids were obtained by the mixed-anhydride method, and characterized with kinetic, electrophoretic and chromatographic techniques (IEF, MCC, CF). The enzyme activities were found to be inversely proportional to the substitution grade but not correlated to the number of carbon atoms of the carboxyalkyl spacer arms. Only the oxidative form (ADH2) of the enzyme was found capable of reacting with theophylline derivatives in the experimental conditions. Enzyme conjugates were resolved into three components, due to the different distribution of theophylline moieties on the surface of the protein, as confirmed by examination of the tryptic patterns of the single components.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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