TFIIH is a multisubunit protein complex that plays an essential role in nucleotide excision repair and transcription of protein-coding genes. Here, we report that TFIIH is also required for ribosomal RNA synthesis in vivo and in vitro. In yeast, pre-rRNA synthesis is impaired in TFIIH ts strains. In a mouse, part of cellular TFIIH is localized within the nucleolus and is associated with subpopulations of both RNA polymerase I and the basal factor TIF-IB. Transcription systems lacking TFIIH are inactive and exogenous TFIIH restores transcriptional activity. TFIIH is required for productive but not abortive rDNA transcription, implying a postinitiation role in transcription. The results provide a molecular link between RNA polymerase I transcription and transcription-coupled repair of active ribosomal RNA genes.

• PMID: 12015980
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Reference Type

Journal Article | Research Support, Non-U.S. Gov't

Authors

Iben S, Tschochner H, Bier M, Hoogstraten D, Hozák P, Egly JM, Grummt I

Primary Lit For

SSL2 | TFB1 | KIN28 | TFB5 | RAD3 | SSL1 | TFB4 | TFB3 | CCL1 | TFB2 | General transcription factor TFIIH complex | ... Show allDNA-directed RNA Polymerase I complex Show fewer

Additional Lit For

RPO21 | RPA43

Review For