Reference: Pardo M, et al. (1999) Two-dimensional analysis of proteins secreted by Saccharomyces cerevisiae regenerating protoplasts: a novel approach to study the cell wall. Yeast 15(6):459-72

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Abstract


Protoplasts of Saccharomyces cerevisiae incubated in regenerating conditions secrete cell wall components in order to allow the biosynthesis of this structure. During the first hours of incubation, many of these are not retained in the forming cell wall but remain in the medium. We have developed a method for collecting the secreted proteins and have analysed these by two-dimensional electrophoresis to obtain a reference map of putative cell wall proteins. Several proteins were identified by microsequencing or immunoblotting; namely, cell wall hydrolytic enzymes, heat shock proteins, glycolytic enzymes and others. Some beta-1,3- and beta-1, 6-glucosylation was detected in the proteins secreted by regenerating protoplasts.

Reference Type
Journal Article | Research Support, Non-U.S. Gov't
Authors
Pardo M, Monteoliva L, Pla J, Sánchez M, Gil C, Nombela C
Primary Lit For
PST1 | YGP1
Additional Lit For
SSA1 | BGL2 | SSB1 | TDH3 | ENO2 | TDH2 | HSP150 | GAS3 | SSB2 | SSA2 | FBA1 ... Show all

Gene Ontology Annotations 1 entry for 1 gene


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Gene/ComplexQualifierGene Ontology TermAnnotation ExtensionEvidenceSourceAssigned On
YGP1involved incell wall assemblyIDASGD2013-08-07
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