ARR1 / YPR199C Overview
- Standard Name
- ARR1 1
- Systematic Name
- YPR199C
- SGD ID
- SGD:S000006403
- Aliases
- ACR1 2 , YAP8 7
- Feature Type
- ORF , Verified
- Description
- Transcriptional activator of the basic leucine zipper (bZIP) family; required for transcription of genes involved in resistance to arsenic compounds; directly binds trivalent arsenic (As(III)) as does K. lactis ortholog, KIYAP8 2 3 4 6
- Name Description
- ARsenicals Resistance 5
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 294
- Mol. Weight (Da)
- 33230.5
- Isoelectric Point
- 8.21
- Median Abundance (molecules/cell)
- 1157
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.
View all ARR1 alleles in SGD search | YeastMine
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Sequence specific DNA-binding transcriptional activator of genes involved in response to arsenic; localizes to nucleus and cytoplasm
View computational annotations
Molecular Function
- Manually Curated
Biological Process
- Manually Curated
- involved in cellular response to arsenic-containing substance (IGI, IMP, HMP)
- involved in positive regulation of transcription by RNA polymerase II (HMP, IMP, IGI)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant has decreased fitness and accumulates arsenite, making it sensitive to various forms of arsenic; heterozygous diploid null is haploinsufficient
Classical Genetics
- null
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
89 total interactions for 75 unique genes
Physical Interactions
- Affinity Capture-RNA: 2
- Affinity Capture-Western: 4
- Co-localization: 1
- Co-purification: 1
- FRET: 1
- PCA: 1
- Two-hybrid: 45
Genetic Interactions
- Dosage Rescue: 5
- Negative Genetic: 17
- Phenotypic Enhancement: 4
- Phenotypic Suppression: 1
- Positive Genetic: 2
- Synthetic Growth Defect: 4
- Synthetic Rescue: 1
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- ARR1 encodes an AP-1 type transcription factor which contains a basic leucine zipper domain (bZIP), and binds to a consensus cis-acting Yap recognition element (YRE; 5'-TTAC/GTAA-3'). Arr1p is the master regulator of the arsenic stress response, mediating transcriptional activation of the ARR2 arsenate reductase and the ARR3 plasma membrane arsenite antiporter, thereby facilitating extrusion of arsenite molecules from the cytoplasm. The activity of Arr1p is primarily regulated at the level of its export from the nucleus. Under normal conditions Arr1p is freely distributed between the cytoplasm and nucleus, but mostly it is localized in the cytosol due to its active export from the nucleus by Crm1p, which binds a nuclear export signal (NES) in the N-terminal cysteine-rich domain (n-CRD) of Arr1p. Arr1p is activated by arsenite, which modifies the sulfhydryl groups of specific cysteines, masking the NES and abolishing its recognition by Crm1p.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.