CHP1 / YPL225W Overview

Standard Name
CHP1 2
Systematic Name
Feature Type
ORF , Verified 1
Chaperone for eEF1A; ribosome-associated chaperone dedicated to facilitating high-level expression of eEF1A; transiently binds nascent GTPase domain of eEF1A as soon as it emerges from ribosome tunnel; forms complex with NAC by binding UBA domain of Egd2p; inactivation results in disturbed proteostasis and Hsf1p activation due to abberant eEF1A biogenesis; overlapping phenotypes support notion that Chp1p functions in same eEF1A biogenesis pathway as chaperone Zpr1p-Aim29p 2
Name Description
CHaPerone 2
Comparative Info
Sequence Details


The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.

CHP1 is located on the left arm of chromosome XVI toward the telomere between translation termination and ribosome biogenesis factor NEW1 and MMT2 mitochondrial metal transporter; coding sequence is 441 nucleotides long with one synonymous SNP
Protein Details


Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.

Chp1p is 146 amino acids long, longer-lived, moderate in abundance; acetylated on 4 lysines, phosphorylated on T35 and S36, sumoylated on K46, ubiquitinylated on 4 lysines; protein abundance increases in response to DNA replication stress
Length (a.a.)
Mol. Weight (Da)
Isoelectric Point
Median Abundance (molecules/cell)
12973 +/- 6464
Half-life (hr)


Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.

View all CHP1 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.

Cytoplasmic protein-folding chaperone for eEF1A; binds ribosomes, nascent peptide-associated complex to facilitate cotranslational 'de novo' protein folding of eEF1A; also binds misfolded eEF1A

View computational annotations

Biological Process

Manually Curated

Cellular Component

Manually Curated
Phenotype Details


Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.

Interaction Details


Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.

Chp1p interacts physically with proteins involved in translation regulation; CHP1 interacts genetically with genes involved in cytoskeleton organization

129 total interactions for 116 unique genes

Physical Interactions

  • Affinity Capture-MS: 18
  • Affinity Capture-RNA: 6
  • Co-localization: 1
  • Co-purification: 1
  • PCA: 5
  • Proximity Label-MS: 1

Genetic Interactions

  • Negative Genetic: 82
  • Positive Genetic: 10
  • Synthetic Growth Defect: 4
  • Synthetic Rescue: 1
Regulation Details


The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.

CHP1 promoter is bound by Bur6p, Reb1p, Stp1p, and Tfc7p in response to heat; CHP1 transcription is regulated by Gcr1p and Reb1p
Expression Details


Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.

Literature Details


All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.