CDC42 / YLR229C Overview

Standard Name
CDC42 1
Systematic Name
Feature Type
ORF , Verified
Small rho-like GTPase; essential for establishment and maintenance of cell polarity; plays a role late in cell fusion via activation of key cell fusion regulator Fus2p; acts with ESCRT proteins at sites of nuclear envelope and ER fission; mutants have defects in the organization of actin and septins; human homolog CDC42 can complement yeast cdc42 mutant 2 3 4 5
Name Description
Cell Division Cycle 1
Comparative Info
Sequence Details


The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.

CDC42 is on the right arm of chromosome XII between replication origin ARS1218 and isoleucine tRNA; dubious ORF YLR230W overlaps CDC42 on the opposite strand; coding sequence is 576 nucleotides long with 2 SNPs, 1 of which leads to an Ala/Thr amino acid polymorphism at residue 189 very near the C-terminus
Protein Details


Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.

Protein is 191 amino acids long with 5 transforming protein P21 ras signature domains; shares several domains with Rho proteins involved in cytoskeleton organization; phosphorylated at 2 residues, ubiquitinylated at 2 other residues
Length (a.a.)
Mol. Weight (Da)
Isoelectric Point
Median Abundance (molecules/cell)
5221 +/- 1690
Half-life (hr)


Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.

cdc42-1 | cdc42-101 | cdc42-102 | cdc42-105 | cdc42-107 | cdc42-109 | cdc42-111 | ... Show all

View all CDC42 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.

GTPase involved in establishment of cell polarity, bud growth, septin ring organization, and mating; involved in regulating mitotic exit, pseudohyphal growth, non-autophagic vacuole fusion, and exocytosis; localizes to the nuclear, vacuolar, and plasma membranes and also to incipient bud sites, sites of polarized growth, and the septin ring

View computational annotations

Molecular Function

Manually Curated

Cellular Component

Manually Curated
Phenotype Details


Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.

Essential gene; conditional mutant arrests as large, unbudded cells and repressible mutant displays a G1 phase cell cycle defect; conditional mutant affects the polarized localization of Sec3p and Sec5p to emerging bud sites, bud tips, and bud necks; mutants with a reduction-of-function allele have a bilateral cell fusion defect; conditional mutant displays increased chromosomal instability; heterozygous diploid shows haploinsufficiency
Interaction Details


Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.

Interacts physically with proteins involved in cytoskeleton organization and mitosis; interacts genetically with genes involved in mitosis and cytoskeleton organization

684 total interactions for 328 unique genes

Physical Interactions

  • Affinity Capture-MS: 12
  • Affinity Capture-RNA: 7
  • Affinity Capture-Western: 31
  • Biochemical Activity: 4
  • Co-fractionation: 2
  • Co-localization: 2
  • Co-purification: 1
  • FRET: 3
  • PCA: 4
  • Reconstituted Complex: 32
  • Two-hybrid: 86

Genetic Interactions

  • Dosage Growth Defect: 3
  • Dosage Lethality: 2
  • Dosage Rescue: 79
  • Negative Genetic: 203
  • Phenotypic Enhancement: 11
  • Phenotypic Suppression: 24
  • Positive Genetic: 52
  • Synthetic Growth Defect: 30
  • Synthetic Lethality: 79
  • Synthetic Rescue: 17
Regulation Details


The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.

Transcription is regulated by Fkh2p, and by Reb1p and Tfc7p in response to heat; protein stability is negatively regulated by Rsp5p
Expression Details


Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.

Summary Paragraph

A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.

Last Updated: 1999-11-24

Literature Details


All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.