Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided.
14 entries for 10 phenotypesIncrease the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.
| Gene | Phenotype | Experiment Type | Mutant Information | Strain Background | Chemical | Details | Reference |
|---|---|---|---|---|---|---|---|
| CDC42 | actin cytoskeleton morphology: abnormal | classical genetics | conditional Allele: cdc42-1 G142S, T189A (G424A, A565G) | Other | Temperature: elevated temperature, 37 °C Details: 80% of cells have a completely unpolarized actin cytoskeleton and 0% have a highly polarized actin cytoskeleton | Adamo JE, et al. (2001) PMID:11706050 | |
| CDC42 | budding pattern: abnormal | classical genetics | conditional Allele: cdc42-1 G142S, T189A (G424A, A565G) | Other | 2% galactose | Treatment: derepressing conditions Details: random budding pattern in ~75% of cells | Johnson DI and Pringle JR (1990) PMID:2164028 |
| CDC42 | budding pattern: abnormal | classical genetics | conditional Allele: cdc42-1 G142S, T189A (G424A, A565G) | Other | 2% glucose | Treatment: repressing conditions Details: random budding pattern in ~35% of cells | Johnson DI and Pringle JR (1990) PMID:2164028 |
| CDC42 | budding pattern: abnormal | homozygous diploid | conditional Allele: cdc42-1 G142S, T189A (G424A, A565G) | Other | 2% galactose | Treatment: derepressing conditions Details: random budding pattern in ~75% of cells | Johnson DI and Pringle JR (1990) PMID:2164028 |
| CDC42 | budding: absent | homozygous diploid | conditional Allele: cdc42-1 G142S, T189A (G424A, A565G) | Other | Temperature: elevated temperature, 36 °C | Adams AE, et al. (1990) PMID:2195038 | |
| CDC42 | cell cycle progression in G1 phase: arrested | homozygous diploid | activation Allele: cdc42-1 G142S, T189A (G424A, A565G) | Other | Temperature: elevated temperature, 36 °C Details: exhibit first cycle arrest as large unbudded cells, that begin nuclear cycles | Adams AE, et al. (1990) PMID:2195038 | |
| CDC42 | cell size: increased | homozygous diploid | conditional Allele: cdc42-1 G142S, T189A (G424A, A565G) | Other | Temperature: elevated temperature, 36 °C | Adams AE, et al. (1990) PMID:2195038 | |
| CDC42 | chromosome/plasmid maintenance: abnormal | large-scale survey | conditional Allele: cdc42-1 | S288C | Assay: gross-chromosomal rearrangement (GCR) assay Temperature: semi-permissive temperature Details: indicative of chromosome instability (CIN); metascore not determined | Stirling PC, et al. (2011) PMID:21552543 | |
| CDC42 | heat sensitivity: increased | homozygous diploid | conditional Allele: cdc42-1 G142S, T189A (G424A, A565G) | Other | Temperature: elevated temperature, 36 °C | Adams AE, et al. (1990) PMID:2195038 | |
| CDC42 | protein/peptide distribution: abnormal Reporter: Sec4p | classical genetics | conditional Allele: cdc42-1 G142S, T189A (G424A, A565G) | Other | Temperature: elevated temperature, 37 °C Details: reduction in the polarization of Sec4p in small budded cells; ~40% of cells display polarized Sec4p staining compared to 90% of small budded wt cells | Adamo JE, et al. (2001) PMID:11706050 |
An interaction is defined as an experimentally observed physical or genetic interaction between two genes. There may be more than one row listed for the same interactor if the interaction between it and the given gene was observed in separate studies. All interactions listed in SGD are curated by BioGRID.
141 entries for 111 genesIncrease the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.
| Interactor | Interactor | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Reference | |
|---|---|---|---|---|---|---|---|---|---|---|
Genetic interactions were considered significant if they had a p-value < 0.05 and an SGA score > 0.16 for positive interactions and SGA score < -0.12 for negative interactions. | CDC42 | BEM4 | cdc42-1, bem4-Δ | Negative Genetic | high-throughput | Bait-Hit | -0.5332 | 1.491E-15 | Costanzo M, et al. (2016) PMID:27708008 | |
Genetic interactions were considered significant if they had a p-value < 0.05 and an SGA score > 0.16 for positive interactions and SGA score < -0.12 for negative interactions. | CDC42 | BEM4 | bem4-Δ, cdc42-1 | Negative Genetic | high-throughput | Hit-Bait | -0.456 | 5.618E-28 | Costanzo M, et al. (2016) PMID:27708008 | |
Genetic interactions were considered significant if they had a p-value < 0.05 and an SGA score > 0.16 for positive interactions and SGA score < -0.12 for negative interactions. | STU1 | CDC42 | stu1-5, cdc42-1 | Positive Genetic | high-throughput | Bait-Hit | 0.224 | 0.0004796 | Costanzo M, et al. (2016) PMID:27708008 | |
Genetic interactions were considered significant if they had a p-value < 0.05 and an SGA score > 0.16 for positive interactions and SGA score < -0.12 for negative interactions. | SHG1 | CDC42 | cdc42-1, shg1-Δ | Positive Genetic | high-throughput | Hit-Bait | 0.1918 | 0.006616 | Costanzo M, et al. (2016) PMID:27708008 | |
Genetic interactions were considered significant if they had a p-value < 0.05 and an SGA score > 0.16 for positive interactions and SGA score < -0.12 for negative interactions. | BFA1 | CDC42 | cdc42-1, bfa1-Δ | Positive Genetic | high-throughput | Hit-Bait | 0.2047 | 0.01132 | Costanzo M, et al. (2016) PMID:27708008 | |
Genetic interactions were considered significant if they had a p-value < 0.05 and an SGA score > 0.16 for positive interactions and SGA score < -0.12 for negative interactions. | SAC1 | CDC42 | cdc42-1, sac1-Δ | Positive Genetic | high-throughput | Hit-Bait | 0.2512 | 2.346E-26 | Costanzo M, et al. (2016) PMID:27708008 | |
Genetic interactions were considered significant if they had a p-value < 0.05 and an SGA score > 0.16 for positive interactions and SGA score < -0.12 for negative interactions. | FUN19 | CDC42 | fun19-Δ, cdc42-1 | Positive Genetic | high-throughput | Bait-Hit | 0.18 | 0.0406 | Costanzo M, et al. (2016) PMID:27708008 | |
Genetic interactions were considered significant if they had a p-value < 0.05 and an SGA score > 0.16 for positive interactions and SGA score < -0.12 for negative interactions. | LIH1 | CDC42 | yjr107w-Δ, cdc42-1 | Positive Genetic | high-throughput | Bait-Hit | 0.1872 | 0.007097 | Costanzo M, et al. (2016) PMID:27708008 | |
Genetic interactions were considered significant if they had a p-value < 0.05 and an SGA score > 0.16 for positive interactions and SGA score < -0.12 for negative interactions. | CDC24 | CDC42 | cdc24-1, cdc42-1 | Negative Genetic | high-throughput | Bait-Hit | -0.5002 | 3.035E-16 | Costanzo M, et al. (2016) PMID:27708008 | |
Genetic interactions were considered significant if they had a p-value < 0.05 and an SGA score > 0.16 for positive interactions and SGA score < -0.12 for negative interactions. | CDC24 | CDC42 | cdc24-11, cdc42-1 | Negative Genetic | high-throughput | Bait-Hit | -0.2708 | 0.0006252 | Costanzo M, et al. (2016) PMID:27708008 |
This diagram displays up to the top 30 positive interactions (green), negative interactions (orange), and phenotypes (blue) that are shared between the given allele (black) and other alleles (purple). The shared interactions or phenotypes can be visualized separately using the radio button at the bottom of the graph.
List of references used specifically to curate the information in Overview section.
Addgene Plasmids | DNASU Plasmids | PlasmID | Thermo Scientific | YGRC
dHITS | FitDB | HIPHOP Chemogenomics | HIP HOP Profiles | PhenoM | PROPHECY | SCMD | ScreenTroll | TheCellVision | Yeast Phenome
BioGRID | CYC2008 | DIP | GeneMANIA | IMP | InterologFinder | ModelArchive | TheCellMap | YeastRC Two-Hybrid