HXK2 / YGL253W Overview


Standard Name
HXK2 1
Systematic Name
YGL253W
SGD ID
SGD:S000003222
Aliases
HEX1 21 , HKB 22 , SCI2
Feature Type
ORF , Verified
Description
Hexokinase isoenzyme 2; phosphorylates glucose in cytosol; predominant hexokinase during growth on glucose; represses expression of HXK1, GLK1, induces expression of its own gene; antiapoptotic; phosphorylation/dephosphorylation at Ser14 by kinase Snf1p, phosphatase Glc7p-Reg1p regulates nucleocytoplasmic shuttling of Hxk2p; functions downstream of Sit4p in control of cell cycle, mitochondrial function, oxidative stress resistance, chronological lifespan; has paralog HXK1 2 3 4 5 6 7
Name Description
HeXoKinase
Paralog
HXK1 5
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Summary
HXK2 is located on the left arm of chromosome VII near the telomere and between FZF1 transcription factor and RTG2 mitochondrial dysfuction sensor; coding sequence is 1461 nucleotides long with 4 synonymous SNPs
Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Length (a.a.)
486
Mol. Weight (Da)
53930.9
Isoelectric Point
4.93
Median Abundance (molecules/cell)
136905 +/- 64001
Half-life (hr)
17.8

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.


HXK2-Δ(K479-A486),S305F | hxk2-(hex1-18) | hxk2-082 | hxk2-104 | hxk2-106 | hxk2-108 | hxk2-109 | ... Show all

View all HXK2 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
Hexokinase, phosphorylates D-hexoses to D-hexose-6-phosphates; involved in cellular metabolism and homeostasis of glucose, fructose and mannose; localized to cytosol, nucleus and mitochondria

View computational annotations

Molecular Function

Manually Curated

Cellular Component

Manually Curated

Pathways


Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
Non-essential gene; null mutant displays reduced growth rate, decreased replicative lifespan and increased apoptosis; null mutation also causes sensitivity to alkaline pH, acetic acid, fluconazole, but increased resistance to caffeine, camptothecin, bleomycin
Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


Summary
The hxk2 null mutant is viable; the null mutant of paralog hxk1 is viable; the hxk2 hxk1 double mutant is inviable or displays a growth defect.

496 total interactions for 325 unique genes

Physical Interactions

  • Affinity Capture-MS: 53
  • Affinity Capture-RNA: 8
  • Affinity Capture-Western: 15
  • Biochemical Activity: 2
  • Co-purification: 2
  • PCA: 3
  • Proximity Label-MS: 1
  • Reconstituted Complex: 10
  • Two-hybrid: 6

Genetic Interactions

  • Negative Genetic: 283
  • Phenotypic Enhancement: 26
  • Phenotypic Suppression: 10
  • Positive Genetic: 51
  • Synthetic Growth Defect: 10
  • Synthetic Lethality: 5
  • Synthetic Rescue: 11
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Regulators
12
Targets
0
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Summary Paragraph

A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.


Last Updated: 2006-11-29

Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
206
Additional
312
Reviews
54

Resources