SEM1 / YDR363W-A Overview


Standard Name
SEM1 1
Systematic Name
YDR363W-A
SGD ID
SGD:S000007235
Aliases
DSS1 10 , HOD1
Feature Type
ORF , Verified
Description
19S proteasome regulatory particle lid subcomplex component; role in Ub-dependent proteolysis and proteasome stability; involved in TREX-2 mediated mRNA export, and in the prevention of transcription-associated genome instability; ubiquitinated by Nedd4-like E3-ligase, Rsp5p; human ortholog DSS1, a BRCA1 binding protein implicated in cancer, complements the yeast null; drives trinucleotide repeat expansion; protein abundance increases in response to DNA replication stress 1 2 3 4 5 6 7 8 9
Name Description
Suppressor of Exocyst Mutations 1
Comparative Info
Integrated model organism details available at the Alliance of Genome Resources website
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Length (a.a.)
89
Mol. Weight (Da)
10372.1
Isoelectric Point
3.92
Median Abundance (molecules/cell)
13352 +/- 7505
Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
Peripheral lid subunit of proteasome regulatory particle involved in ubiquitin-dependent protein degradation; involved in messenger RNA (mRNA) export from nucleus, filamentous growth, histone deubiquitination and DNA trinucleotide repeats

View computational annotations

Molecular Function

Manually Curated

Cellular Component

Manually Curated

Complex

Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.


Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
Non-essential gene; null mutant exhibits sensitivity to heat and UV, resistance to 4-NQO, and decreased nuclear export of poly(A)+ RNA; in large-scale studies, null mutant shows reduced competitive fitness in several different media, displays sensitivity to acid pH and desiccation, and has shortened telomeres; null mutant exhibits a respiratory growth defect at 37 deg C; null mutant has altered resistance to a variety of chemicals
Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


940 total interactions for 497 unique genes

Physical Interactions

  • Affinity Capture-MS: 85
  • Affinity Capture-RNA: 7
  • Affinity Capture-Western: 35
  • Biochemical Activity: 1
  • Co-crystal Structure: 2
  • Co-purification: 3
  • PCA: 3
  • Reconstituted Complex: 1
  • Two-hybrid: 1

Genetic Interactions

  • Dosage Growth Defect: 1
  • Dosage Rescue: 7
  • Negative Genetic: 576
  • Phenotypic Enhancement: 6
  • Phenotypic Suppression: 3
  • Positive Genetic: 143
  • Synthetic Growth Defect: 22
  • Synthetic Lethality: 32
  • Synthetic Rescue: 12
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Regulators
6
Targets
0
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
44
Additional
58
Reviews
21

Resources