PDC2 / YDR081C Overview


Standard Name
PDC2 1
Systematic Name
YDR081C
SGD ID
SGD:S000002488
Feature Type
ORF , Verified
Description
Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions 2 3 4 5 6 7 8
Name Description
Pyruvate DeCarboxylase 1
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Length (a.a.)
925
Mol. Weight (Da)
103934.4
Isoelectric Point
6.39
Median Abundance (molecules/cell)
1938 +/- 1296
Half-life (hr)
8.8

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.


View all PDC2 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
Transcription factor that regulates transcription by RNA Pol II, thiamine biosynthesis, and glycolytic fermentation to ethanol

View computational annotations

Molecular Function

Manually Curated

Cellular Component

Manually Curated
Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


408 total interactions for 395 unique genes

Physical Interactions

  • Affinity Capture-MS: 8
  • Affinity Capture-RNA: 7
  • Co-purification: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 1

Genetic Interactions

  • Dosage Rescue: 2
  • Negative Genetic: 280
  • Positive Genetic: 105
  • Synthetic Rescue: 3
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Summary
PDC2 encodes a transcriptional activator without overall similarity to known transcription factor families. Pdc2p is essential for transcriptional activation of genes involved in thiamin pyrophosphate (TPP; also known as ThDP) biosynthesis, such as PHO3, THI4, and THI20, in response to thiamin deprivation. Pdc2p also activates transcription of the PDC1 and PDC5 genes, encoding pyruvate decarboxylases involved in alcoholic fermentation. Pdc2p can bind independently to target promoters, but many of its thiamin biosynthetic gene targets are shared with those of the transcription factor Thi2p, and binding of Pdc2p to promoters is enhanced in the presence of Pdc2p and another regulatory protein, Thi3p, that binds both Pdc2p and Thi2p. In the absence of thiamin, Thi3p interaction with Pdc2p and Thi2p allows them to activate transcription, possibly by a conformational change that unmasks their activation domains. In the presence of TPP (i.e., under thiamin-replete conditions), Thi3p binding to TPP releases it from Pdc2p and Thi2p, de-activating them. Pdc2p activates transcription of PDC5 independently of Thi2p and Thi3p, but in a thiamine-dependent manner. In contrast, Pdc2p activates transcription of PDC1 constitutively.
Regulators
1
Targets
14
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
13
Additional
26
Reviews
11

Resources