THI2 / YBR240C Overview
- Standard Name
- THI2 1
- Systematic Name
- YBR240C
- SGD ID
- SGD:S000000444
- Aliases
- PHO6 , phoF 7
- Feature Type
- ORF , Verified
- Description
- Transcriptional activator of thiamine biosynthetic genes; interacts with regulatory factor Thi3p to control expression of thiamine biosynthetic genes with respect to thiamine availability; acts together with Pdc2p to respond to thiaminediphosphate demand, possibly as related to carbon source availability; zinc finger protein of the Zn(II)2Cys6 type 1 2 3 4 5 6
- Name Description
- THIamine metabolism
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 450
- Mol. Weight (Da)
- 51835.4
- Isoelectric Point
- 8.63
- Median Abundance (molecules/cell)
- 1226
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all THI2 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- DNA binding transcription activator involved in thiamine biosynthesis
View computational annotations
Molecular Function
- Manually Curated
- enables RNA polymerase II cis-regulatory region sequence-specific DNA binding (IDA)
- enables zinc ion binding (RCA)
Biological Process
- Manually Curated
- involved in positive regulation of thiamine biosynthetic process (IMP)
- involved in positive regulation of transcription by RNA polymerase II (IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
Classical Genetics
- null
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
40 total interactions for 35 unique genes
Physical Interactions
- Affinity Capture-MS: 1
- Co-localization: 1
- Co-purification: 1
- Reconstituted Complex: 1
- Two-hybrid: 1
Genetic Interactions
- Dosage Rescue: 3
- Negative Genetic: 25
- Positive Genetic: 6
- Synthetic Lethality: 1
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- THI2 encodes a transcription factor that is a member of the C6 zinc finger class, containing a DNA binding domain also known as the Zn2Cys6 binuclear zinc cluster or zinc knuckle. Thi2p is required for transcriptional activation of a subset of genes involved in thiamin pyrophosphate (TPP; also known as ThDP) biosynthesis in response to thiamin deprivation. The targets of Thi2p include PHO3, THI4, and THI20. Thi2p can bind independently to target promoters, but its targets are shared with those of the transcription factor Pdc2p, and binding of Thi2p to promoters is enhanced in the presence of Pdc2p and another regulatory protein, Thi3p, that binds both Thi2p and Pdc2p. In the absence of thiamin, Thi3p interaction with Thi2p and Pdc2p allows them to activate transcription, possibly by a conformational change that unmasks their activation domains. In the presence of TPP (i.e., under thiamin-replete conditions), Thi3p binding to TPP releases it from Thi2p and Pdc2p, de-activating them.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.