CDC7 / YDL017W Overview

Standard Name
CDC7 1
Systematic Name
LSD6 , SAS1 41
Feature Type
ORF , Verified
S/T kinase and catalytic subunit of DDK (Dbf4-dependent kinase); required for origin firing and replication initiation; phosphorylates MCM subunits and Cdc45 to drive assembly of the pre-IC and the CMG helicase; maintains Cse4p at kinetochores through phosphorylation to regulate chromosome segregation, regulates pre-meiotic replication, meiotic DSB formation, monopolar attachment of homologs during MI through monopolin recruitment and middle meiotic gene expression; HsCDC7 and DBF4 complement 2 3 4 5 7 8 9 10 11 12 13 14 15 16 17 18
Name Description
Cell Division Cycle 6
Comparative Info
Sequence Details


The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.

Protein Details


Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.

Length (a.a.)
Mol. Weight (Da)
Isoelectric Point
Median Abundance (molecules/cell)
1562 +/- 677
Half-life (hr)


Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.

View all CDC7 alleles in SGD search

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.

Protein serine/threonine kinase and catalytic subunit of the Dbf4-dependent protein kinase (DDK) complex; required for the initiation of mitotic and pre-meiotic DNA replication and ds break repair-induced replication, phosphorylating subunits of the MCM helicase complex, Cdc45p and Pol1p; regulates formation of the replication pre-initiation complex and the CMG complex; negative regulator of mitotic exit; positive regulator of meiosis I, promoting transcription of middle-late genes including NDT80 via phosphorylation of repressor Sum1p, meiotic recombination via phosphorylation of Rec107p, homologous chromosome segregation via phosphorylation of monopolin subunit Lrs4p, and recruitment of monopolin to kinetochores

View computational annotations

Molecular Function

Manually Curated

Cellular Component

Manually Curated


Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.

Phenotype Details


Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.

Essential gene; conditional mutant arrests at the G1/S phase transition of the cell cycle as large budded cells with nuclei situated at the mother-bud neck; conditional mutants display chromosomal instability with elevated loss and gross rearrangement at semi-permissive temperatures; sporulation is absent in diploids at the non-permissive temperature with cells arresting in meiosis after pre-meiotic DNA replication and SPB duplication but before spindle formation in meiosis I and SC formation; mutants are sensitive to DNA damaging agents
Interaction Details


Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.

860 total interactions for 479 unique genes

Physical Interactions

  • Affinity Capture-MS: 33
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 26
  • Biochemical Activity: 53
  • Co-localization: 1
  • Co-purification: 5
  • FRET: 1
  • Protein-peptide: 1
  • Reconstituted Complex: 19
  • Two-hybrid: 31

Genetic Interactions

  • Dosage Growth Defect: 3
  • Dosage Lethality: 6
  • Dosage Rescue: 7
  • Negative Genetic: 454
  • Phenotypic Enhancement: 9
  • Phenotypic Suppression: 13
  • Positive Genetic: 72
  • Synthetic Growth Defect: 76
  • Synthetic Lethality: 23
  • Synthetic Rescue: 25
Regulation Details


The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.

Expression Details


Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.

Summary Paragraph

A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.

Last Updated: 1999-04-12

Literature Details


All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.