HMLALPHA1 / YCL066W Overview
- Standard Name
- HMLALPHA1
- Systematic Name
- YCL066W
- SGD ID
- SGD:S000000571
- Aliases
- ALPHA1 7
- Feature Type
- ORF , Verified
- Description
- Silenced copy of ALPHA1 at HML; ALPHA1 encodes a transcriptional coactivator involved in the regulation of mating-type alpha-specific gene expression 1 2 3 4 5 6
- Name Description
- HoMothallism Left 7
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 175
- Mol. Weight (Da)
- 20428.7
- Isoelectric Point
- 10.59
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Transcription coactivator involved in regulation of transcription by RNA polymerase II; localizes to nucleus
View computational annotations
Molecular Function
- Manually Curated
- enables DNA binding, bending (ISA)
- enables transcription coactivator activity (ISA)
Biological Process
- Manually Curated
- involved in regulation of mating-type specific transcription, DNA-templated (ISA)
- involved in regulation of transcription by RNA polymerase II (ISA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
No phenotype data available.
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
7 total interactions for 7 unique genes
Physical Interactions
- Biochemical Activity: 1
- Co-crystal Structure: 1
- Co-localization: 1
- Reconstituted Complex: 1
- Two-hybrid: 3
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- HMLALPHA1 is a silenced copy of the ALPHA1 gene located in one of two cryptic copies (HMLalpha and HMRa) of the mating-type cassette, both of which serve as donors during the recombinational process that allows mating-type switching. Alpha1p is a transcriptional co-activator that acts as a heterodimer with Mcm1p and targets it to the promoters of a set of alpha mating-type specific genes, including alpha-factor mating pheromone and the Ste2p 'a' mating-type specific transmembrane receptor. The pheromone facilitates conjugation of opposite haploid types into 'a'/alpha diploids. Alpha1p is expressed in haploids of the alpha mating type, and is required for the expression of alpha-specific genes. In diploids, expression of ALPHA1 is turned of by the action of the very stable a1-alpha2 corepressor, which turns off haploid-specific genes and allows expression of diploid-specific genes. Alpha1p is rapidly degraded via ubiquitin-mediated proteolysis by the proteasome. Both HML and HMR are surrounded by distinct silencer sequences that interact with several factors including the four Sir proteins, silencer binding proteins, histones, Rap1p, and several chromatin modifiers, which create short regions of transcriptionally silent heterochromatin.
- Regulators
- 7
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2000-08-17
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.