CDC24 / YAL041W Overview
- Standard Name
- CDC24 1
- Systematic Name
- YAL041W
- SGD ID
- SGD:S000000039
- Aliases
- CLS4 7
- Feature Type
- ORF , Verified
- Description
- Guanine nucleotide exchange factor (GEF) for Cdc42p; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress; thermosensitivity of the cdc24-4 mutant in the presence of sorbitol is functionally complemented by human CDC42 2 3 5 6
- Name Description
- Cell Division Cycle 4
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- CDC24 is located on the left arm of chromosome I between ERV46 COPII-coated vesicle protein and CLN3 G1 cyclin; coding sequence is 2565 nucleotides long with 7 nonsynonymous SNPs, 19 synonymous SNPs
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Relocalizes from nucleus to cytoplasm upon DNA replication stress
- Length (a.a.)
- 854
- Mol. Weight (Da)
- 96946.8
- Isoelectric Point
- 8.72
- Median Abundance (molecules/cell)
- 3501 +/- 680
- Half-life (hr)
- 7.4
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.
View all CDC24 alleles in SGD search | YeastMine
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Rho guanine nucleotide exchange factor for Cdc42p; involved in cellular bud site selection, septin ring organization, and regulation of mitotic exit; localizes to the nucleus, cytoplasm, incipient bud sites, and sites of polarized growth
View computational annotations
Molecular Function
- Manually Curated
Biological Process
- Manually Curated
- involved in cellular bud site selection (IMP)
- involved in chemotropism (IMP)
- acts upstream of or within pheromone-dependent signal transduction involved in conjugation with cellular fusion (IGI, IMP)
- involved in protein localization to cell cortex (IMP)
- involved in regulation of exit from mitosis (IMP)
- involved in response to pheromone triggering conjugation with cellular fusion (IMP)
- involved in septin ring organization (IMP)
Cellular Component
- Manually Curated
- part of Cdc24p-Far1p-Gbetagamma complex (IDA, IPI)
- located in cellular bud neck (IDA)
- located in cellular bud tip (IDA)
- located in cytoplasm (IDA, HDA)
- located in incipient cellular bud site (IDA)
- located in mating projection tip (IDA)
- located in nucleus (HDA, IDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Essential gene; conditional mutants are arrested in cell cycle as large unbudded cells with abnormal nuclear morphology; show mating defects and abnormal shmoo formation even at permissive temperatures
Classical Genetics
- conditional
- axial budding pattern: decreased
- budding: absent
- cell cycle progression: arrested
- cell size: increased
- cellular morphology: abnormal
- chitin deposition: increased
- heat sensitivity: increased
- mating projection morphology: abnormal
- nuclear morphology: abnormal
- protein/peptide distribution: abnormal
- shmoo formation: absent
- sporulation: decreased
- viability: decreased
- unspecified
- null
- overexpression
- null
- reduction of function
- repressible
- conditional
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
1001 total interactions for 716 unique genes
Physical Interactions
- Affinity Capture-MS: 42
- Affinity Capture-RNA: 5
- Affinity Capture-Western: 25
- Biochemical Activity: 3
- Co-crystal Structure: 1
- Co-fractionation: 2
- Co-localization: 1
- PCA: 6
- Protein-peptide: 1
- Reconstituted Complex: 22
- Two-hybrid: 53
Genetic Interactions
- Dosage Lethality: 2
- Dosage Rescue: 33
- Negative Genetic: 664
- Phenotypic Enhancement: 3
- Phenotypic Suppression: 8
- Positive Genetic: 88
- Synthetic Growth Defect: 6
- Synthetic Lethality: 22
- Synthetic Rescue: 14
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 7
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.