UBI4 / YLL039C Overview

Standard Name
UBI4 1
Systematic Name
SCD2 11 , UB14 5
Feature Type
ORF , Verified
Ubiquitin; becomes conjugated to proteins, marking them for selective degradation via ubiquitin-26S proteasome system; essential for cellular stress response; encoded as polyubiquitin precursor comprised of 5 head-to-tail repeats; number of repeats is evolutionarily unstable within and between yeast species; protein abundance increases in response to DNA replication stress; implicated as modulator of replicative lifespan and meiotic recombination 1 2 3 4 5 6 7 8 9 10
Name Description
Ubiquitin 1
Comparative Info
Sequence Details


The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.

Protein Details


Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.

Protein abundance increases in response to DNA replication stress
Length (a.a.)
Mol. Weight (Da)
Isoelectric Point
Median Abundance (molecules/cell)
4541 +/- 1755


Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.

ubi4* | ubi4-A46G | ubi4-A46S | ubi4-A46S,I61T | ubi4-A46S,L50P | ubi4-D21A | ubi4-D32A | ... Show all

View all UBI4 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.

Ubiquitin, a protein tag covalently attached to proteins to target them to selective degradation by ubiquitin-dependent proteasome-mediated protein catabolic system; localized to the cytoplasm

View computational annotations

Molecular Function

Manually Curated

Biological Process

Manually Curated

Cellular Component

Manually Curated
Phenotype Details


Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.

Non-essential gene; null mutant is sensitive to various stressors including: elevated temperature, zinc deficiency, nitrogen starvation, oxidative stress, desiccation, canavanine addition and hydrostatic pressure; null mutant displays decreased spore germination, decreased survival in stationary phase, decreased competitive fitness in various growth media, and is an inositol auxotroph; homozygous diploid null mutant has a sporulation defect
Interaction Details


Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.

971 total interactions for 530 unique genes

Physical Interactions

  • Affinity Capture-MS: 83
  • Affinity Capture-RNA: 6
  • Affinity Capture-Western: 57
  • Biochemical Activity: 54
  • Co-crystal Structure: 27
  • Co-fractionation: 2
  • Co-localization: 2
  • Co-purification: 2
  • Far Western: 4
  • FRET: 4
  • PCA: 1
  • Protein-peptide: 10
  • Reconstituted Complex: 187
  • Two-hybrid: 30

Genetic Interactions

  • Dosage Growth Defect: 2
  • Dosage Rescue: 52
  • Negative Genetic: 374
  • Phenotypic Enhancement: 4
  • Phenotypic Suppression: 15
  • Positive Genetic: 30
  • Synthetic Growth Defect: 13
  • Synthetic Lethality: 2
  • Synthetic Rescue: 10
Regulation Details


The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.

Expression Details


Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.

Literature Details


All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.