TPS1 / YBR126C Overview


Standard Name
TPS1 1 2
Systematic Name
YBR126C
SGD ID
SGD:S000000330
Aliases
BYP1 17 , CIF1 15 , FDP1 18 , GGS1 19 , GLC6 20 , TSS1 16
Feature Type
ORF , Verified
Description
Synthase subunit of trehalose-6-P synthase/phosphatase complex; synthesizes the storage carbohydrate trehalose, which is critically important for survival of long-term desiccation; also found in a monomeric form; expression is induced by the stress response and repressed by the Ras-cAMP pathway; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid; required for pseudohyphal growth 3 4 5 6 7 8 9 10
Name Description
Trehalose-6-Phosphate Synthase
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Summary
Protein abundance increases in response to DNA replication stress
Length (a.a.)
495
Mol. Weight (Da)
56136.2
Isoelectric Point
6.0
Median Abundance (molecules/cell)
20541 +/- 7422
Half-life (hr)
9.0

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.


View all TPS1 alleles in SGD search

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
Alpha,alpha-trehalose phosphate synthase subunit of the alpha,alpha-trehalose-phosphate synthase complex; involved in biosynthesis of trehalose and cellular responses to oxidative stress, heat and dessication

View computational annotations

Molecular Function

Manually Curated

Biological Process

Manually Curated

Cellular Component

Manually Curated

Complex

Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.


Pathways


Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
Non-essential gene; null mutant shows decreased innate thermotolerance and higher sensitivity to desiccation; null mutation results in reduced accumulation of glycogen and trehalose, and lower ethanol tolerance; in systematic studies null mutant has small cell size, abnormally elongated buds, reduced respiratory growth rate; decreased biofilm formation and decreased competitive fitness
Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


686 total interactions for 523 unique genes

Physical Interactions

  • Affinity Capture-MS: 94
  • Affinity Capture-RNA: 5
  • Co-purification: 1
  • PCA: 2
  • Proximity Label-MS: 4
  • Reconstituted Complex: 1
  • Two-hybrid: 9

Genetic Interactions

  • Dosage Growth Defect: 1
  • Dosage Lethality: 1
  • Dosage Rescue: 12
  • Negative Genetic: 396
  • Phenotypic Enhancement: 17
  • Phenotypic Suppression: 10
  • Positive Genetic: 85
  • Synthetic Growth Defect: 19
  • Synthetic Lethality: 10
  • Synthetic Rescue: 19
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Regulators
18
Targets
0
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Summary Paragraph

A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.


Last Updated: 2005-08-04

Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
139
Additional
202
Reviews
53

Resources