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  • Phenotype: pexophagy: decreased
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Phenotype: pexophagy: decreased


Observable
pexophagy
Qualifier
decreased

Annotations 7 entries for 7 genes


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.

GenePhenotypeExperiment TypeMutant InformationStrain BackgroundChemicalDetailsReference
ATG17pexophagy: decreased
Reporter: Pot1p
classical geneticsnull
Allele: atg17-Δ
OtherCheong H, et al. (2005) PMID:15901835
EGD1pexophagy: decreased
classical genetics null
Allele: egd1-Δ
S288CTemperature: glycerol-containing
Details: partial reduction in pexophagy compared to wt in glycerol grown cells at 72 hours; assayed using Pot1-mCherry, a peroxisomal matrix marker that is transported to the vacuole where it is processed during pexophagy; free mCherry signals are reduced to 54% of wt levels, as quantitated by western blot
Tian Y and Okamoto K (2024) PMID:38177147
EGD2pexophagy: decreased
classical genetics null
Allele: egd2-Δ
S288CTemperature: glycerol-containing
Details: partial reduction in pexophagy compared to wt in glycerol grown cells at 72 hours; assayed using Pot1-mCherry, a peroxisomal matrix marker that is transported to the vacuole where it is processed during pexophagy; free mCherry signals are reduced to 68% of wt levels, as quantitated by western blot
Tian Y and Okamoto K (2024) PMID:38177147
RAS2pexophagy: decreased
classical genetics activation
Allele: RAS2-val19

G19V; impaired GTPase activity; dominant allele locked in the activated state; analogous to G12V in oncogenic forms of human Ras

S288CTreatment: glucose starvation and rapamycin treatment
Boutouja F and Platta HW (2020) PMID:33202661
SEC15pexophagy: decreased
classical genetics conditional
Allele: sec15-1
OtherMedia: nitrogen starvation
Temperature: elevated temperature, 37 °C
Details: reduction in pexophagy based on decreased accumulation of free GFP in cells expressing Pot1-GFP after incubation overnight in oleate-containing media followed by transfer to nitrogen-starvation media at the non-permissive temperature
Singh S, et al. (2019) PMID:31103773
TPS1pexophagy: decreased
classical genetics null
Allele: tps1-Δ
S288CMedia: glucose-containing nitrogen starvation media
Details: defect in pexophagy, measured as a reduction in the processing of Pex11-GFP under nitrogen starvation conditions
Kim B, et al. (2021) PMID:32240040
TPS2pexophagy: decreased
classical genetics null
Allele: tps2-Δ
S288CMedia: glucose-containing nitrogen starvation media
Details: defect in pexophagy, measured as a reduction in the processing of Pex11-GFP under nitrogen starvation conditions
Kim B, et al. (2021) PMID:32240040
Showing 1 to 7 of 7 entries
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