TOP1 / YOL006C Overview
- Standard Name
- TOP1 1
- Systematic Name
- YOL006C
- SGD ID
- SGD:S000005366
- Aliases
- MAK1 1 , MAK17 15
- Feature Type
- ORF , Verified
- Description
- Topoisomerase I; nuclear enzyme that relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone; relaxes both positively and negatively supercoiled DNA; functions in replication, transcription, and recombination; role in processing ribonucleoside monophosphates in genomic DNA into irreversible single-strand breaks; enzymatic activity and interaction with Nsr1p are negatively regulated by polyphosphorylation 1 2 3 4 5 6
- Name Description
- TOPoisomerase 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 769
- Mol. Weight (Da)
- 90008.4
- Isoelectric Point
- 9.22
- Median Abundance (molecules/cell)
- 4130 +/- 2517
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all TOP1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- DNA topoisomerase type I that unwinds DNA; involved in chromatin organization, chromosome condensation, DNA replication, recombination and transcription; subunit of the replication fork protection complex which stabilizes stalled replication forks and is thought to be involved in coordinating leading- and lagging-strand synthesis and in replication checkpoint signaling
View computational annotations
Molecular Function
- Manually Curated
Biological Process
- Manually Curated
- involved in chromatin organization (IMP)
- involved in DNA strand elongation involved in DNA replication (IMP)
- involved in DNA topological change (IDA, IMP)
- involved in mitotic chromosome condensation (IMP)
- involved in nuclear migration (IMP)
- involved in rDNA heterochromatin formation (IMP)
- involved in regulation of mitotic recombination (IMP)
- involved in regulation of transcription by RNA polymerase II (IMP)
- involved in rRNA transcription (IGI)
- involved in transcription elongation by RNA polymerase II (IMP)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant has an increased respiratory but decreased fermentative growth rate; null mutant is resistant to topo I inhibitors but sensitive to topo II inhibitors and DNA damaging agents; null mutant displays decreased resistance to hyperosmotic stress, alkaline pH and metals, but increased resistance to oxidative stress and increased chronological lifespan; homozygous diploid null mutant has increased mitotic chromosome loss, increased loss of heterozygosity and a sporulation defect; overexpression results in a decreased growth rate and enhanced invasive growth
Classical Genetics
- null
- apoptosis: decreased
- chromosome/plasmid maintenance: abnormal
- gamma ray resistance: increased
- metal resistance: decreased
- protein/peptide accumulation: decreased
- protein/peptide modification: absent
- protein/peptide modification: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- RNA accumulation: increased
- vegetative growth: decreased rate
- vegetative growth: delayed
- reduction of function
- conditional
- null
- alkaline pH resistance: decreased
- chemical compound accumulation: abnormal
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- chronological lifespan: increased
- competitive fitness: decreased
- desiccation resistance: decreased
- endocytosis: decreased
- haploproficient
- hyperosmotic stress resistance: decreased
- metal resistance: decreased
- oxidative stress resistance: decreased
- oxidative stress resistance: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- respiratory growth: increased rate
- sporulation: decreased
- stress resistance: decreased
- vegetative growth: decreased rate
- viable
- overexpression
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
1049 total interactions for 653 unique genes
Physical Interactions
- Affinity Capture-MS: 61
- Affinity Capture-RNA: 5
- Affinity Capture-Western: 12
- Biochemical Activity: 2
- Reconstituted Complex: 1
- Two-hybrid: 12
Genetic Interactions
- Dosage Growth Defect: 1
- Dosage Lethality: 1
- Dosage Rescue: 2
- Negative Genetic: 399
- Phenotypic Enhancement: 30
- Phenotypic Suppression: 36
- Positive Genetic: 127
- Synthetic Growth Defect: 279
- Synthetic Lethality: 38
- Synthetic Rescue: 43
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 2
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 1999-11-09
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.