Phenotype Help

HUB1 / YNR032C-A Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided.


Summary
Non-essential gene; null mutant has slow growth, abnormal budding pattern, defective shmoo formation and decreased mating

Annotations

A phenotype is defined as an observable (e.g., apoptosis) and a qualifier (e.g., increased). There may be more than one row with the same phenotype if that phenotype was observed in separate studies or in different conditions, strains, alleles, etc.

20 entries for 15 phenotypes


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PhenotypeExperiment TypeMutant InformationStrain BackgroundChemicalDetailsReference
bipolar budding pattern: abnormal
homozygous diploidnull
Allele: hub1-Δ
W303Details: defective bud site selection resulting in decreased budding from the distal tip and increased proximal and random budding
Dittmar GA, et al. (2002) PMID:11923536
competitive fitness: increased
competitive growth

fitness profiling using complete deletion alleles

null
Allele: hub1-Δ
S288CMedia: minimal medium
Details: Relative fitness score: 1.009
Breslow DK, et al. (2008) PMID:18622397
competitive fitness: increased
systematic mutation setnull
Allele: hub1-Δ
S288CMedia: rich medium (YPD) w/6% ethanol, ETH
Qian W, et al. (2012) PMID:23103169
heat sensitivity: increased
homozygous diploid, systematic mutation set

pooled screening for fitness defects at 37 deg C relative to 30 deg C

null
Allele: hub1-Δ
S288CTemperature: elevated temperature, 37 °C
Sinha H, et al. (2008) PMID:18780730
mating efficiency: decreased
classical geneticsnull
Allele: hub1-Δ
W303Dittmar GA, et al. (2002) PMID:11923536
mating efficiency: decreased
classical genetics activation
Allele: hub1-Δ72Δ73

deletion of the C-terminal tyrosine and leucine residues 72-73

W303Details: mutant show little or no activity in a mating assay
Dittmar GA, et al. (2002) PMID:11923536
mating efficiency: normal
classical genetics unspecified
Allele: hub1-Δ73

deletion of the C-terminal leucine 72

W303Details: mutant is not functionally impaired in the mating assay
Dittmar GA, et al. (2002) PMID:11923536
mating projection morphology: abnormal
classical geneticsnull
Allele: hub1-Δ
W303Details: shmooing defects; form rounded or peanut-shaped morphology
Dittmar GA, et al. (2002) PMID:11923536
protein secretion: increased
Reporter: heterologous B. licheniformis alpha-amylase
homozygous diploid, systematic mutation setnull
Allele: hub1-Δ
S288CHoshida H, et al. (2013) PMID:23306636
protein/peptide distribution: abnormal
Reporter: Hbt1-GFP
classical genetics null
Allele: hub1-Δ
W303Treatment: α-factor, 5 μM
Details: Hbt1p, normally localized within or proximal to the mating projection in α-factor treated cells that had elaborated a mating projection, becomes delocalized in the null mutant
Dittmar GA, et al. (2002) PMID:11923536
Showing 1 to 10 of 20 entries

Shared Phenotypes

This diagram displays phenotype observables (purple squares) that are shared between the given gene (yellow circle) and other genes (gray circles) based on the number of phenotype observables shared (adjustable using the slider at the bottom).


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