Phenotype Help

CSA1 / YPR174C Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided.


Summary
Non-essential gene; null mutant shows decreased competitive fitness on minimal medium and has an altered free amino acid profile; null mutant has decreased resistance to hyperosmotic stress, caffeine, fenpropimorph, and bleomycin, but increased tolerance to rapamycin and the toxic dipeptide Ala-Eth; homozygous diploid null mutant shows decreased tolerance to chromium and the carcinogen benzo[a]pyrene; reduction-of-function mutants show abnormal protein/peptide modification and localization

Annotations

A phenotype is defined as an observable (e.g., apoptosis) and a qualifier (e.g., increased). There may be more than one row with the same phenotype if that phenotype was observed in separate studies or in different conditions, strains, alleles, etc.

20 entries for 10 phenotypes


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.

PhenotypeExperiment TypeMutant InformationStrain BackgroundChemicalDetailsReference
chemical compound accumulation: abnormal
systematic mutation setnull
Allele: csa1-Δ
S288C alpha-amino acidDetails: Significantly altered free amino acid profile (X^2- test p < 0.01)
Mülleder M, et al. (2016) PMID:27693354
competitive fitness: decreased
competitive growth

fitness profiling using complete deletion alleles

null
Allele: csa1-Δ
S288CMedia: minimal medium
Details: Relative fitness score: 0.98
Breslow DK, et al. (2008) PMID:18622397
hyperosmotic stress resistance: decreased
systematic mutation setnull
Allele: csa1-Δ
S288C1 M sodium chlorideYoshikawa K, et al. (2009) PMID:19054128
nutrient utilization: decreased
systematic mutation setnull
Allele: csa1-Δ
S288C His-LeuMedia: cultured in minimal medium containing histidine, lysine, uracil and tryptophan
Details: decreased ability to utilize the His-Leu dipeptide resulting in poor growth relative to the wildtype control
Cai H, et al. (2006) PMID:16361226
protein/peptide accumulation: decreased
Reporter: Ptr2p-GFP
classical geneticsnull
Allele: csa1-Δ
S288CCai H, et al. (2006) PMID:16361226
protein/peptide modification: abnormal
Reporter: Cdc5-Venus
classical genetics null
Allele: csa1-Δ
W303Details: strong decrease in Cdc5 signal at spindle pole body during metaphase; detected as nucleoplasmic signal
Örd M, et al. (2020) PMID:32553169
protein/peptide modification: abnormal
Reporter: Cdc5-Venus
classical genetics reduction of function
Allele: csa1-(ypr174c(S170A))
W303Details: strong decrease in Cdc5 signal at spindle pole body during metaphase; detected as nucleoplasmic signal
Örd M, et al. (2020) PMID:32553169
protein/peptide modification: abnormal
Reporter: Cdc5-Venus
classical genetics reduction of function
Allele: csa1-(ypr174c(AP))

S69A, S158A, S170A, S175A, S211A

W303Details: strong decrease in Cdc5 signal at spindle pole body during metaphase; detected as nucleoplasmic signal
Örd M, et al. (2020) PMID:32553169
protein/peptide modification: abnormal
Reporter: Cdc5-Venus
classical genetics reduction of function
Allele: csa1-(ypr174c(pxf))
W303Details: slight decrease in Cdc5 accumulation at the spindle pole bodies; wild type levels of nuclear localization
Örd M, et al. (2020) PMID:32553169
protein/peptide modification: abnormal
Reporter: NES-Clb3-GFP
classical genetics reduction of function
Allele: csa1-(ypr174c(pxf))

P190A, P193A, F195A

W303Details: loss of Clb3-GFP accumulation in the nuclear envelope; cytoplasmic puncta observed
Örd M, et al. (2020) PMID:32553169
Showing 1 to 10 of 20 entries

Shared Phenotypes

This diagram displays phenotype observables (purple squares) that are shared between the given gene (yellow circle) and other genes (gray circles) based on the number of phenotype observables shared (adjustable using the slider at the bottom).


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Click on a gene or phenotype observable name to go to its specific page within SGD; drag any of the gene or observable objects around within the visualization for easier viewing; click “Reset” to automatically redraw the diagram; filter the genes that share observable terms with the given gene by the number of terms they share by clicking anywhere on the slider bar or dragging the tab to the desired filter number.


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