AIM44 / YPL158C Overview
- Standard Name
- AIM44 1
- Systematic Name
- YPL158C
- SGD ID
- SGD:S000006079
- Aliases
- GPS1 4
- Feature Type
- ORF , Verified
- Description
- Regulator of Cdc42p and Rho1p; regulates AMR closure through Hof1p; inhibits Cdc42-dependent Cla4 activation at the division site, to prevent budding in the old bud neck; recruits Nis1p and Nba1p to the division site with Nap1 and the Rax1p-Rax2p dependent inheritance of Nis1p and Nba1p to bud scars to prevent division site repolarization; keeps Rho1p at the division site after AMR contraction to control secondary septum formation; relocalizes from bud neck to cytoplasm upon replication stress 2 3 4 5 6
- Name Description
- Altered Inheritance rate of Mitochondria 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 758
- Mol. Weight (Da)
- 84843.0
- Isoelectric Point
- 6.63
- Median Abundance (molecules/cell)
- 238 +/- 18
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all AIM44 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Protein with a role in assembly of the septum between dividing cells during cytokinesis; localized to the bud neck and bud tip
View computational annotations
Molecular Function
- Manually Curated
- enables molecular function unknown (ND)
Biological Process
- Manually Curated
- involved in maintenance of protein location (IMP)
- involved in positive regulation of mitotic actomyosin contractile ring contraction (IMP)
- involved in protein localization to cell division site after cytokinesis (IMP)
- involved in regulation of protein localization to cell division site (IMP)
- involved in secondary cell septum biogenesis (IGI, IMP)
Cellular Component
- Manually Curated
- located in cell division site (IDA)
- is active in cellular bud neck (IMP, IDA, HDA)
- located in cellular bud neck septin collar (IDA)
- located in cellular bud neck split septin rings (IDA)
- located in cellular bud tip (HDA)
- located in nuclear inner membrane (IMP)
- located in plasma membrane (IDA, IMP)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant displays decreased rates of fermentative and respiratory growth, and decreased competitive fitness in various media; null mutant accumulates mother cells that retain a connection to two or more buds, displays a thin secondary septum, a reduction in the accumulation of Rho1p at the bud neck after actomyosin ring (AMR) contraction, and an abnormal reaccumulation of Cdc42p at bud neck after AMR contraction, all indicative of abnormal coordination of cytokinesis and polarity establishment; null mutant displays an increased frequency of spontaneous mitochondrial genome loss; null mutant has increased chitin deposition, decreased endocytosis and decreased peroxisomal protein transport; overexpression results in decreased filamentation and invasive growth in the presence of short-chain alcohols and increased invasive growth in nitrogen-sufficient medium
Classical Genetics
- unspecified
- null
- budding: abnormal
- cell death: increased
- chitin deposition: increased
- cytokinesis: abnormal
- cytokinesis: decreased
- growth in exponential phase: decreased rate
- protein/peptide accumulation: decreased
- protein/peptide accumulation: increased
- protein/peptide distribution: abnormal
- protein/peptide distribution: decreased
- protein/peptide modification: delayed
- protein/peptide modification: increased
- resistance to chemicals: decreased
- septum formation: abnormal
- overexpression
- reduction of function
- null
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- colony appearance: abnormal
- competitive fitness: decreased
- endocytosis: decreased
- mitochondrial genome maintenance: abnormal
- peroxisomal transport: decreased
- replicative lifespan: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- respiratory growth: decreased rate
- sporulation: normal
- toxin resistance: decreased
- vegetative growth: decreased rate
- viable
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
877 total interactions for 507 unique genes
Physical Interactions
- Affinity Capture-MS: 6
- Affinity Capture-RNA: 8
- Affinity Capture-Western: 5
- Co-localization: 1
- PCA: 2
- Reconstituted Complex: 3
- Two-hybrid: 17
Genetic Interactions
- Dosage Lethality: 1
- Dosage Rescue: 3
- Negative Genetic: 608
- Phenotypic Suppression: 2
- Positive Genetic: 206
- Synthetic Growth Defect: 4
- Synthetic Lethality: 5
- Synthetic Rescue: 6
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 4
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.