Phenotype Help

FRE5 / YOR384W Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided.



Annotations

A phenotype is defined as an observable (e.g., apoptosis) and a qualifier (e.g., increased). There may be more than one row with the same phenotype if that phenotype was observed in separate studies or in different conditions, strains, alleles, etc.

12 entries for 10 phenotypes


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.

PhenotypeExperiment TypeMutant InformationStrain BackgroundChemicalDetailsReference
chemical compound excretion: increased
homozygous diploid, systematic mutation set

quantification of extracellular glutathione at stationary phase

null
Allele: fre5-Δ
S288C glutathioneTreatment: SD medium
Details: Fold elevation of glutathione excretion: 2
Perrone GG, et al. (2005) PMID:15509654
competitive fitness: increased
competitive growth

fitness profiling using complete deletion alleles

null
Allele: fre5-Δ
S288CMedia: minimal medium
Details: Relative fitness score: 1.015
Breslow DK, et al. (2008) PMID:18622397
heat sensitivity: decreased
systematic mutation set

high throughput heat ramp cell death assay

null
Allele: fre5-Δ
S288CTemperature: elevated temperature
Details: ramp temperature from ambient to 30 deg C immediately, hold 1 min at 30 deg C, ramp temperature from 30 deg C to 62 deg C over 20 min, return to ambient
Teng X, et al. (2011) PMID:21814286
invasive growth: increased
homozygous diploid, systematic mutation setoverexpressionSigma1278bMedia: nitrogen-sufficient medium
Details: agar invasion score: 0.96; enhanced pseudohyphal growth, based on elevated invasiveness
Shively CA, et al. (2013) PMID:23410832
oxidative stress resistance: decreased
classical genetics null
Allele: fre5-Δ
S288C10 mM hydrogen peroxideDetails: significant decrease in colony formation in the null mutant of genes with UUC-enriched transcripts, after acute exposure to hydrogen peroxide induced oxidative stress
Endres L, et al. (2020) PMID:32053677
resistance to chemicals: decreased
competitive growthoverexpressionS288C1.3 nM sirolimusDetails: sirolimus is rapamycin
Butcher RA, et al. (2006) PMID:16415861
resistance to chemicals: decreased
systematic mutation setnull
Allele: fre5-Δ
S288C CG-1521Gaupel AC, et al. (2014) PMID:24968945
resistance to chemicals: decreased
heterozygous diploid, systematic mutation set null
Allele: fre5-Δ
S288C2.5% dimethyl sulfoxideLum PY, et al. (2004) PMID:14718172
resistance to chemicals: increased
large-scale survey

variomic library screening of ~10,000 alleles for each gene

unspecifiedS288C100 ng/ml cycloheximideHuang Z, et al. (2013) PMID:23416056
utilization of carbon source: decreased rate
systematic mutation set

prototrophic version of yeast deletion collection

null
Allele: fre5-Δ
S288C galactoseVanderSluis B, et al. (2014) PMID:24721214
Showing 1 to 10 of 12 entries

Shared Phenotypes

This diagram displays phenotype observables (purple squares) that are shared between the given gene (yellow circle) and other genes (gray circles) based on the number of phenotype observables shared (adjustable using the slider at the bottom).


Reset

Click on a gene or phenotype observable name to go to its specific page within SGD; drag any of the gene or observable objects around within the visualization for easier viewing; click “Reset” to automatically redraw the diagram; filter the genes that share observable terms with the given gene by the number of terms they share by clicking anywhere on the slider bar or dragging the tab to the desired filter number.


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