SFL1 / YOR140W Overview


Standard Name
SFL1 1
Systematic Name
YOR140W
SGD ID
SGD:S000005666
Feature Type
ORF , Verified
Description
Transcriptional repressor and activator; involved in repression of flocculation-related genes, and activation of stress responsive genes; has direct role in INO1 transcriptional memory; negatively regulated by cAMP-dependent protein kinase A subunit Tpk2p; premature stop codon (C1430T, Q477-stop) in SK1 background is linked to the aggressively invasive phenotype of SK1 relative to BY4741 (S288C) 1 2 3 4 5 6 7
Name Description
Suppressor gene for FLocculation 1
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Length (a.a.)
766
Mol. Weight (Da)
83351.7
Isoelectric Point
9.13
Median Abundance (molecules/cell)
1234 +/- 1212
Half-life (hr)
9.2

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.


View all SFL1 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
Transcriptional repressor and activator; involved in positive regulation of autophagy

View computational annotations

Cellular Component

Manually Curated
Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


450 total interactions for 364 unique genes

Physical Interactions

  • Affinity Capture-MS: 15
  • Affinity Capture-RNA: 8
  • Affinity Capture-Western: 5
  • Biochemical Activity: 1
  • Co-localization: 1
  • Reconstituted Complex: 2
  • Two-hybrid: 3

Genetic Interactions

  • Dosage Rescue: 4
  • Negative Genetic: 331
  • Phenotypic Enhancement: 3
  • Phenotypic Suppression: 29
  • Positive Genetic: 35
  • Synthetic Growth Defect: 8
  • Synthetic Lethality: 4
  • Synthetic Rescue: 1
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Summary
SFL1 encodes a winged helix-turn-helix transcription factor of the E2F family. Sfl1p binds sequences of the form 5'-ATAGAAGAA-3' and represses flocculation-related genes, and is required for normal cell-surface assembly during vegetative growth. Sfl1p negatively regulates FLO11 through interference by transcription of the overlapping noncoding RNA ICR1, for which Sfl1p is required. FLO11 is in a transcriptionally permissive state when ICR1 is repressed by the transcription factor Flo8p. Competition between Flo8p and Sfl1p for binding to the FLO11 promoter determines whether ICR1 is transcribed, which keeps FLO11 silenced, or repressed, which allows FLO11 transcription. FLO10 is also regulated by Sfl1p and Flo8p, via a mechanism that involves cis-interfering ncRNAs encoded upstream of FLO10 that likely control gene expression through promoter occlusion. Sfl1p is present at the promoters of Cyc8p-Tup1p-repressible genes, and appears to recruit the general corepressor Cyc8p-Tup1p that inhibits the transcription of many diversely regulated genes. Sfl1p may also interact with Srb/mediator proteins to inhibit transcription by the RNA polymerase II holoenzyme. Phosphorylation of Sfl1p by protein kinase A prohibits dimerization and DNA-binding by Sfl1p.
Regulators
13
Targets
14
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Summary Paragraph

A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.


Last Updated: 2005-05-03

Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
38
Additional
42
Reviews
19

Resources