RAS1 / YOR101W Overview

Standard Name
RAS1 1
Systematic Name
Feature Type
ORF , Verified
GTPase involved in G-protein signaling in adenylate cyclase activation; plays a role in regulation of cell proliferation; localized to the plasma membrane; activated by increased levels of glycolysis intermediate fructose-1,6-bisphosphate; homolog of mammalian RAS proto-oncogenes; relative distribution to the nucleus increases upon DNA replication stress; RAS1 has a paralog, RAS2, that arose from the whole genome duplication 1 2 3 4 5 6
Name Description
homologous to RAS proto-oncogene 1
RAS2 5
Comparative Info
Sequence Details


The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.

RAS1/YOR101W is located on the right arm of chromosome XV between CRC1 mitochondrial inner membrane carnitine transporter and OST2 oligosaccharyltransferase subunit; coding sequence is 930 nucleotides long with 28 SNPs, 9 of which cause amino acid polymorphisms; RAS1 has a paralog, RAS2, that arose from the whole genome duplication
Protein Details


Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.

Ras1p is 309 amino acids long, shorter-lived, low in abundance; contains 5 transforming protein P21 ras signatures; palmitoylated on C305, ubiquitinylated on 7 lysines, phosphorylated on 17 residues; relative distribution to the nucleus increases upon DNA replication stress
Length (a.a.)
Mol. Weight (Da)
Isoelectric Point
Median Abundance (molecules/cell)
2813 +/- 977
Half-life (hr)


Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.

View all RAS1 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.

GTPase involved in the G-protein coupled receptor signaling pathway via activation of adenylate cyclase activity; has roles in replicative cell aging and protein localization to the bud neck; localizes to the plasma membrane

View computational annotations

Molecular Function

Manually Curated

Cellular Component

Manually Curated
Phenotype Details


Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.

Non-essential gene; null mutant has decreased chronological and increased replicative lifespan; null mutant has a decreased rate of growth on various nitrogen sources, decreased starvation resistance, and increased competitive fitness in minimal medium; overexpression increases invasive growth, and a dominant interfering allele results in a growth defect in the absence of cAMP
Disease Details


Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.

Yeast RAS1 is homologous to human KRAS and HRAS, and has been used to study mutations found in patients with colorectal cancer, urinary bladder cancer, and Costello syndrome

Manually Curated


Interaction Details


Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.

Ras1p interacts physically with proteins involved in cell cycle regulation, RAS1 interacts genetically with genes involved in mitotic cell cycle; the ras1 null mutant is viable, the null mutant of paralog ras2 is viable, the ras1 ras2 double mutant is inviable or displays a growth defect

140 total interactions for 95 unique genes

Physical Interactions

  • Affinity Capture-MS: 10
  • Affinity Capture-RNA: 6
  • Affinity Capture-Western: 1
  • Biochemical Activity: 1
  • Co-purification: 1
  • PCA: 2
  • Reconstituted Complex: 4
  • Two-hybrid: 6

Genetic Interactions

  • Dosage Growth Defect: 1
  • Dosage Lethality: 3
  • Dosage Rescue: 19
  • Negative Genetic: 43
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 2
  • Positive Genetic: 11
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 6
  • Synthetic Rescue: 19
Regulation Details


The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.

RAS1 promoter is bound by Fkh2p, Med4p, Msn2p, Nhp6ap, Rgr1p, and Ume6p in response to heat; RAS1 transcription is regulated by Sfp1p in response to stress
Expression Details


Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.

Summary Paragraph

A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.

Last Updated: 2024-07-03

Literature Details


All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.