CKA2 / YOR061W Overview


Standard Name
CKA2 1
Systematic Name
YOR061W
SGD ID
SGD:S000005587
Aliases
YOR29-12
Feature Type
ORF , Verified
Description
Alpha' catalytic subunit of casein kinase 2 (CK2); CK2 is a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; protein abundance increases in response to DNA replication stress; regulates Fkh1p-mediated donor preference during mating-type switching 1 2 3 4 5
Name Description
Casein Kinase Alpha' subunit 1
Comparative Info
Integrated model organism details available at the Alliance of Genome Resources website
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Length (a.a.)
339
Mol. Weight (Da)
39409.0
Isoelectric Point
8.76
Median Abundance (molecules/cell)
6548 +/- 1915
Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
Protein serine/threonine kinase subunit of the protein kinase CK2 complex, also a subunit of the UTP-C subcomplex of 90S preribosome; involved in regulation of transcription by RNA polymerases I and III, chronological cell aging, and cellular responses to osmotic stress and DNA damage; found in nearly every subcellular compartment

View computational annotations

Molecular Function

Manually Curated

Cellular Component

Manually Curated

Complex

Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.


Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
Non-essential gene; null mutation causes ionic stress sensitivity, increases resistance to benomyl and extends replicative lifespan; in systematic studies mutants show increased sensitivity to a variety of chemicals, including Calcofluor White, Congo Red, wortmannin, geldanamycin, nigericin, rapamycin, caspofungin, fluconazole, tunicamycin
Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


617 total interactions for 400 unique genes

Physical Interactions

  • Affinity Capture-MS: 165
  • Affinity Capture-RNA: 7
  • Affinity Capture-Western: 26
  • Biochemical Activity: 43
  • Co-purification: 3
  • PCA: 5
  • Reconstituted Complex: 10
  • Two-hybrid: 10

Genetic Interactions

  • Dosage Growth Defect: 10
  • Dosage Lethality: 11
  • Dosage Rescue: 11
  • Negative Genetic: 159
  • Phenotypic Enhancement: 7
  • Phenotypic Suppression: 24
  • Positive Genetic: 100
  • Synthetic Growth Defect: 10
  • Synthetic Lethality: 6
  • Synthetic Rescue: 10
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Regulators
2
Targets
1
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
86
Additional
69
Reviews
30

Resources