SHE4 / YOR035C Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided.

Summary

SHE4/YOR035C is a non-essential gene in reference strain S288C; deletion is viable but causes pronounced heat sensitivity (37–39°C), slower vegetative and fermentative growth, and reduced competitive fitness. Mutants show broad morphology and trafficking defects: enlarged cells with abnormal buds and shape, vacuolar and ER abnormalities (exacerbated by salt), elevated chitin deposition, altered protein distribution, impaired endocytosis, and nuclear transport defects; HU-induced filamentous growth and budding patterns are abnormal. Genome stability and stress responses are altered, with decreased mutation frequency, mitotic recombination, transposon transposition, UV and desiccation resistance, and reduced respiratory growth in copper, alongside impaired nitrogen utilization (urea, glutamate), abnormal amino acid handling (global alpha–amino‑acid imbalance; reduced threonine, isoleucine, glutamine, asparagine), and reduced tellurite accumulation. she4Δ is broadly drug‑sensitive, especially to cell wall and membrane stressors and genotoxins (Calcofluor White, Congo Red, caspofungin, hydroxyurea, caffeine, bleomycin, camptothecin), solvents and acids (ethanol and other alcohols, acetaldehyde, DMSO, oleic acid; acetic, lactic, HCl, sulfuric), monensin, quinine, and zymolyase, and shows reduced resistance to manganese. Conversely, resistance is increased to methyl methanesulfonate, yttrium and gadolinium salts, paraquat (oxidative stress), and low‑zinc conditions. Toxin responses are mixed: killer toxin resistance is increased, whereas sensitivity to Aβ42, trichothecin, and lactoferricin is elevated.

Annotations

A phenotype is defined as an observable (e.g., apoptosis) and a qualifier (e.g., increased). There may be more than one row with the same phenotype if that phenotype was observed in separate studies or in different conditions, strains, alleles, etc.

Shared Phenotypes

This diagram displays phenotype observables (purple squares) that are shared between the given gene (yellow circle) and other genes (gray circles) based on the number of phenotype observables shared (adjustable using the slider at the bottom).

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