Phenotype Help

AUS1 / YOR011W Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided.


Summary
Non-essential gene; null mutant displays reduced sterol uptake and decreased anaerobic growth; null mutant displays decreased starvation resistance, decreased utilization of arginine as a nitrogen source but increased competitive fitness in minimal media; null mutant is sensitive to treatment with nickel sulfate; null mutant displays increased survival following a ramped heat stimulus

Annotations

A phenotype is defined as an observable (e.g., apoptosis) and a qualifier (e.g., increased). There may be more than one row with the same phenotype if that phenotype was observed in separate studies or in different conditions, strains, alleles, etc.

9 entries for 7 phenotypes


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PhenotypeExperiment TypeMutant InformationStrain BackgroundChemicalDetailsReference
chemical compound accumulation: decreased
classical geneticsnull
Allele: aus1-Δ
W303 cholesterolDetails: reduced uptake of exogenous cholesterol
Wilcox LJ, et al. (2002) PMID:12077145
chemical compound accumulation: decreased
classical genetics null
Allele: aus1-Δ
S288C cholesterolTreatment: anaerobic
Details: five times less cholesterol uptake under anaerobiosis than wild-type
Papay M, et al. (2020) PMID:31830308
chemical compound accumulation: decreased
classical genetics null
Allele: aus1-Δ
S288C campesterol, sitosterol, stigmastanolTreatment: anaerobic
Details: three times less uptake of plant sterols under anaerobic conditions
Papay M, et al. (2020) PMID:31830308
competitive fitness: increased
competitive growth

fitness profiling using complete deletion alleles

null
Allele: aus1-Δ
S288CMedia: minimal medium
Details: Relative fitness score: 1.012
Breslow DK, et al. (2008) PMID:18622397
heat sensitivity: decreased
systematic mutation set

high throughput heat ramp cell death assay

null
Allele: aus1-Δ
S288CTemperature: elevated temperature
Details: ramp temperature from ambient to 30 deg C immediately, hold 1 min at 30 deg C, ramp temperature from 30 deg C to 62 deg C over 20 min, return to ambient
Teng X, et al. (2011) PMID:21814286
resistance to chemicals: decreased
systematic mutation setnull
Allele: aus1-Δ
S288C nickel sulfateArita A, et al. (2009) PMID:19917080
starvation resistance: decreased
heterozygous diploid, competitive growth

pooled mutant strains were subjected to low nutrient stress, then dead cells were separated and their mutations identified

null
Allele: aus1-Δ
S288CDavey HM, et al. (2012) PMID:22356628
utilization of nitrogen source: decreased rate
systematic mutation set

prototrophic version of yeast deletion collection

null
Allele: aus1-Δ
S288C arginineVanderSluis B, et al. (2014) PMID:24721214
viable
systematic mutation setnull
Allele: aus1-Δ
S288CGiaever G, et al. (2002) PMID:12140549
Showing 1 to 9 of 9 entries

Shared Phenotypes

This diagram displays phenotype observables (purple squares) that are shared between the given gene (yellow circle) and other genes (gray circles) based on the number of phenotype observables shared (adjustable using the slider at the bottom).


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