CSE2 / YNR010W Overview

Standard Name
CSE2 1
Systematic Name
Feature Type
ORF , Verified
Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; component of the Middle domain of mediator; required for regulation of RNA polymerase II activity; relocalizes to the cytosol in response to hypoxia 2 3 4 5
Name Description
Chromosome SEgregation 1
Comparative Info
Sequence Details


The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.

Protein Details


Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.

Relocalizes to the cytosol in response to hypoxia
Length (a.a.)
Mol. Weight (Da)
Isoelectric Point
Median Abundance (molecules/cell)
1895 +/- 436
Half-life (hr)


Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.

View all CSE2 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.

Subunit of the core mediator complex that acts as RNA polymerase II coactivator involved in preinitiation complex assembly; required for regulation of transcription by RNA polymerase II; localized to the nucleus; also found in the cytoplasm under hypoxia

View computational annotations

Molecular Function

Manually Curated

Cellular Component

Manually Curated


Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.

Phenotype Details


Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.

Non-essential gene; null mutation causes slow growth, elevated rate of chromosome loss, increased cold and heat sensitivity; in systematic studies mutations confer decreased chronological lifespan, decreased endocytosis, increased sensitivity to multiple chemicals, including caffeine, rapamycin, MMS, hygromycin B, azoles
Interaction Details


Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.

1073 total interactions for 576 unique genes

Physical Interactions

  • Affinity Capture-MS: 72
  • Affinity Capture-RNA: 5
  • Affinity Capture-Western: 56
  • Biochemical Activity: 1
  • Co-purification: 2
  • Reconstituted Complex: 6
  • Two-hybrid: 3

Genetic Interactions

  • Dosage Rescue: 1
  • Negative Genetic: 706
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 2
  • Positive Genetic: 156
  • Synthetic Growth Defect: 35
  • Synthetic Lethality: 19
  • Synthetic Rescue: 8
Regulation Details


The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.

CSE2 encodes a subunit of the Mediator complex, a large, 25-subunit protein complex that mediates between transcription activators and the general transcription machinery. Mediator serves as a target of many regulatory signals: it binds activation domains of various activators and the C-terminal domain (CTD) of the largest subunit of RNA polymerase II (Pol II). By bringing them together, Mediator enhances basal transcription, facilitates activated transcription, and stimulates CTD phosphorylation. The Mediator subunits are arranged in four structurally distinct modules, named Head, Middle, Tail, and Kinase, with Cse2p, also known as Med9p, as a component of the Middle module. A specific role of Cse2p remains to be elucidated. Mutations in CSE2 have been shown to have a positive effect on transcription of 175 genes, and negative effect on 209 genes. However, rather than pointing to specific regulatory functions of Cse2p, these observations may reflect a more general impact of changes in the Mediator structure that alter its interactions with transcriptional activators and/or Pol II.
Expression Details


Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.

Literature Details


All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.