Phenotype Help

ATO2 / YNR002C Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided.


Summary
Non-essential gene; dominant negative mutation results in acetic acid sensitivity; null mutant has decreased competitive fitness in minimal medium and heterozygous null has decreased resistance to starvation; null mutant has an increased replicative lifespan; overexpression results in increased colony sectoring, indicative of chromosomal instability

Annotations

A phenotype is defined as an observable (e.g., apoptosis) and a qualifier (e.g., increased). There may be more than one row with the same phenotype if that phenotype was observed in separate studies or in different conditions, strains, alleles, etc.

15 entries for 9 phenotypes


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PhenotypeExperiment TypeMutant InformationStrain BackgroundChemicalDetailsReference
colony sectoring: increased
classical geneticsoverexpressionS288CAssay: quantitative chromosome transmission fidelity (qCTF) assay
Details: >1.5 fold increase in mini-chromosome loss relative to wt, indicative of chromosome instability (CIN)
Zhu J, et al. (2015) PMID:25823586
competitive fitness: decreased
competitive growth

fitness profiling using complete deletion alleles

null
Allele: ato2-Δ
S288CMedia: minimal medium
Details: Relative fitness score: 0.961
Breslow DK, et al. (2008) PMID:18622397
heat sensitivity: decreased
systematic mutation set

high throughput heat ramp cell death assay

null
Allele: ato2-Δ
S288CTemperature: elevated temperature
Details: ramp temperature from ambient to 30 deg C immediately, hold 1 min at 30 deg C, ramp temperature from 30 deg C to 62 deg C over 20 min, return to ambient
Teng X, et al. (2011) PMID:21814286
replicative lifespan: increased
classical geneticsnull
Allele: ato2-Δ
S288CMcCormick MA, et al. (2015) PMID:26456335
replicative lifespan: increased
large-scale survey null
Allele: ato2-Δ
S288CDetails: increase in average replicative lifespan (RLS); assayed by single-cell measurement of RLS using a high-throughput microfluidic Yeast Replicator platform
Ölmez TT, et al. (2023) PMID:38012152
resistance to chemicals: decreased
systematic mutation setnull
Allele: ato2-Δ
S288C22 mg/ml amitroleAlamgir M, et al. (2010) PMID:20691087
resistance to chemicals: decreased
homozygous diploid, systematic mutation setnull
Allele: ato2-Δ
S288C benzo[a]pyreneO'Connor ST, et al. (2012) PMID:23403841
resistance to chemicals: decreased
classical geneticsdominant negative
Allele: ATO2-G258D

G258D; trans-dominant

S288C30 mM acetic acidGentsch M, et al. (2007) PMID:17233767
resistance to chemicals: decreased
classical geneticsdominant negative
Allele: ATO2-L74Q

L74Q; trans-dominant

S288C30 mM acetic acidGentsch M, et al. (2007) PMID:17233767
resistance to chemicals: normal
classical geneticsunspecified
Allele: ATO2-L74Q,Δ258end

L74Q and C-terminal truncation 258-end

S288C30 mM acetic acidGentsch M, et al. (2007) PMID:17233767
Showing 1 to 10 of 15 entries

Shared Phenotypes

This diagram displays phenotype observables (purple squares) that are shared between the given gene (yellow circle) and other genes (gray circles) based on the number of phenotype observables shared (adjustable using the slider at the bottom).


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