MCK1 / YNL307C Overview

Standard Name
MCK1 1
Systematic Name
CMS1 1 24 , YPK1 25
Feature Type
ORF , Verified
Dual-specificity S/T and tyrosine protein kinase; spindle position checkpoint component that works in parallel with Kin4p to prevent mitotic exit network (MEN) activation by the Cdc14 early anaphase release (FEAR) network; roles in chromosome segregation, meiotic entry, genome stability, phosphorylation-dependent degradation of Rcn1p and Cdc6p, inhibition of protein kinase A, transcriptional regulation, inhibition of RNAPIII, calcium stress and inhibition of Clb2p-Cdc28p after nuclear division 1 2 3 4 5 6 7 8 9
Name Description
Meiosis and Centromere regulatory Kinase 1
YGK3 9
Comparative Info
Sequence Details


The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.

MCK1 has a paralog, YGK3, that arose from the whole genome duplication
Protein Details


Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.

Length (a.a.)
Mol. Weight (Da)
Isoelectric Point
Median Abundance (molecules/cell)
8487 +/- 3428
Half-life (hr)


Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.

View all MCK1 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.

Protein serine/threonine/tyrosine kinase involved in protein phosphorylation and breakdown; cyclin-binding protein; implicated in double-strand break repair via nonhomologous end joining, meiotic nuclear division, and mitotic chromosome segregation; involved in in ascospore formation and the cellular response to heat and salt stress

View computational annotations

Biological Process

Manually Curated

Cellular Component

Manually Curated
Phenotype Details


Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.

Non-essential gene; null mutant has a decreased growth rate, is thermosensitive and has reduced innate thermotolerance; null displays elevated rates of mitotic chromosome loss at semi-permissive temperatures and in the presence of benomyl; homozygous null mutant has delayed and reduced sporulation, with defects in ascus maturation; null mutant has a reduced critical cell size at START, delayed mitotic exit and elevated Clb2p-Cdc28p activity; null mutant is sensitive to DNA damaging agents, ionic, oxidative, and hyperosmotic stress; heterozygous null is haploinsufficient; overexpression increases filamentous growth and results in bud elongation
Interaction Details


Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.

The mck1 null mutant is viable; the null mutant of paralog ygk3 is viable; the mck1 ygk3 double mutant displays a synthetic growth defect.

935 total interactions for 628 unique genes

Physical Interactions

  • Affinity Capture-MS: 69
  • Affinity Capture-RNA: 8
  • Affinity Capture-Western: 6
  • Biochemical Activity: 119
  • Co-localization: 1
  • Co-purification: 1
  • PCA: 2
  • Proximity Label-MS: 1
  • Reconstituted Complex: 7
  • Two-hybrid: 8

Genetic Interactions

  • Dosage Growth Defect: 38
  • Dosage Lethality: 4
  • Dosage Rescue: 19
  • Negative Genetic: 433
  • Phenotypic Enhancement: 13
  • Phenotypic Suppression: 5
  • Positive Genetic: 50
  • Synthetic Growth Defect: 115
  • Synthetic Lethality: 17
  • Synthetic Rescue: 19
Regulation Details


The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.

Expression Details


Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.

Summary Paragraph

A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.

Last Updated: 2000-10-02

Literature Details


All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.