Phenotype Help

NRK1 / YNL129W Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided.


Summary
NRK1/YNL129W is a non-essential gene; null mutants are viable but grow slowly and have decreased competitive fitness, decreased prion formation, and abnormal vacuolar morphology; overexpression slows growth

Annotations

A phenotype is defined as an observable (e.g., apoptosis) and a qualifier (e.g., increased). There may be more than one row with the same phenotype if that phenotype was observed in separate studies or in different conditions, strains, alleles, etc.

19 entries for 10 phenotypes


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.

PhenotypeExperiment TypeMutant InformationStrain BackgroundChemicalDetailsReference
chemical compound accumulation: increased
homozygous diploid null
Allele: nrk1-Δ
S288C cobalt(2+)Treatment: cobalt dichloride, 1 mM
Details: cobalt sensitive null mutant accumulates cobalt after treatment with CoCl2 for 12 hrs
Zhao YY, et al. (2020) PMID:31904504
chemical compound accumulation: increased
homozygous diploid null
Allele: nrk1-Δ
S288C reactive oxygen speciesTreatment: cobalt dichloride, 1 mM
Details: increased intracellular accumulation of ROS in cells treated with CoCl2 for 12 hrs; possible defect in scavenging oxidative stress
Zhao YY, et al. (2020) PMID:31904504
chemical compound accumulation: increased
homozygous diploid null
Allele: nrk1-Δ
S288C zinc(2+)Treatment: zinc dichloride, 3 mM
Details: zinc sensitive null mutant accumulates Zn2+ after treatment with ZnCl2 for 2 hrs
Zhao YY, et al. (2020) PMID:31836620
chemical compound accumulation: increased
homozygous diploid null
Allele: nrk1-Δ
S288C reactive oxygen speciesTreatment: zinc dichloride, 3 mM
Details: increased intracellular accumulation of ROS in cells treated with 3 mM ZnCl2 for 2 hrs
Zhao YY, et al. (2020) PMID:31836620
chemical compound accumulation: increased
homozygous diploid null
Allele: nrk1-Δ
S288C reactive oxygen speciesTreatment: zinc dichloride, 8 mM
Details: increased intracellular accumulation of ROS in cells treated with 8 mM ZnCl2 for 5 hrs
Zhao YY, et al. (2020) PMID:31836620
chemical compound accumulation: increased
homozygous diploid null
Allele: nrk1-Δ
S288C reactive oxygen speciesTreatment: sodium dodecyl sulfate, 0.015 %
Details: increased accumulation of ROS relative to wt after treatment for 2hr with SDS
Cao C, et al. (2020) PMID:32066383
chemical compound excretion: increased
classical geneticsnull
Allele: nrk1-Δ
S288C N-ribosylnicotinamideLu SP and Lin SJ (2011) PMID:21349851
competitive fitness: decreased
competitive growth

fitness profiling using complete deletion alleles

null
Allele: nrk1-Δ
S288CMedia: minimal medium
Details: Relative fitness score: 0.955
Breslow DK, et al. (2008) PMID:18622397
haploinsufficient
heterozygous diploid, competitive growth

genome-wide fitness profiling

null
Allele: nrk1-Δ
S288CMedia: turbidostat growth in FPM medium
Details: Relative growth score: -0.0038
Pir P, et al. (2012) PMID:22244311
prion formation: decreased
Reporter: [GAR+] prion
systematic mutation setnull
Allele: nrk1-Δ
S288CDetails: reduced [GAR+] induction upon exposure to Staphylococcus hominis
Jarosz DF, et al. (2014) PMID:25171409
Showing 1 to 10 of 19 entries

Shared Phenotypes

This diagram displays phenotype observables (purple squares) that are shared between the given gene (yellow circle) and other genes (gray circles) based on the number of phenotype observables shared (adjustable using the slider at the bottom).


Reset

Click on a gene or phenotype observable name to go to its specific page within SGD; drag any of the gene or observable objects around within the visualization for easier viewing; click “Reset” to automatically redraw the diagram; filter the genes that share observable terms with the given gene by the number of terms they share by clicking anywhere on the slider bar or dragging the tab to the desired filter number.

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Resources


VPS34