Protein Help

SFB2 / YNL049C Protein

Protein abundance data, domains, shared domains with other proteins, protein sequence retrieval for various strains, sequence-based physico-chemical properties, protein modification sites, and external identifiers for the protein.


Aliases
ISS1 2
Protein Product
COPII subunit SFB2
Feature Type
ORF , Verified
Paralog
SEC24 4

AlphaFold Protein Structure

AlphaFold, developed by DeepMind, is an AI program that accurately predicts protein structures from amino acid sequences, enabling visualization of protein conformations. The predicted structures can be accessed through the Protein Data Bank (PDB) and AlphaFold Protein Structure Database.


Model Confidence

Very high
Confident
Low
Very low

Experimental Data

Contains experimentally-derived protein half-life data obtained using stable isotope labeling by amino acids (SILAC) coupled with mass spectrometry. This section also contains protein abundance data for both untreated and treated cells obtained from over 20 studies. These data have been normalized and converted to a common unit of molecules per cell.


Protein Half Life

Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through its pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

ExperimentResultReference
half-life11.0 hrChristiano R, et al. (2014)

Protein Abundance

Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through its pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

Abundance (molecules/cell)MediaTreatmentTreatment timeFold ChangeVisualizationStrain backgroundOriginal ReferenceReference
2926SDuntreatedconfocal microscopy evidenceS288CChong YT, et al. (2015)Ho B, et al. (2018)
2938SD0.2 M hydroxyurea160 minconfocal microscopy evidenceS288CChong YT, et al. (2015)Ho B, et al. (2018)
1722SD200 ng/ml sirolimus700 min0.59confocal microscopy evidenceS288CChong YT, et al. (2015)Ho B, et al. (2018)
4146YEPDuntreatedflow cytometry evidenceS288CDavidson GS, et al. (2011)Ho B, et al. (2018)
3034YEPDcell quiescence7 dflow cytometry evidenceS288CDavidson GS, et al. (2011)Ho B, et al. (2018)
Showing 1 to 5 of 20 entries

Domains and Classification - S288C

Collection of computationally identified domains and motifs, as determined by InterProScan analysis; includes protein coordinates for the domain, a domain Description, a Source and corresponding accession ID, and the number of S. cerevisiae genes that share the same domain.


Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through its pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

Gene Protein Coordinates Accession ID Description Source No. of Genes with Domain

Domain Locations

Visual representation of the locations of the domains within the protein, as listed in the Domains and Classification table. Each row displays the domain(s) derived from a different Source, with domains color-coded according to this Source.

Scroll over a domain to view its exact coordinates and its Description.

Shared Domains

This diagram displays domains (colored squares) shared between the given protein (yellow circle) and other proteins (gray circles); the domains are color-coded according to their source, as displayed in the Domain Locations table, above.

Reset

Click on a gene or domain name to go to its specific page within SGD; drag any of the gene or domain objects around within the visualization for easier viewing; click “Reset” to automatically redraw the diagram.

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.


sfb2-Δ

View all SFB2 alleles in SGD search

Sequence

Protein sequence for the given gene in S288C and other strains, when available. Use the pull-down menu under "Strain" to select the sequence for a specific strain. The displayed sequence can be downloaded in FASTA format as a .txt file. Amino acids displayed in blue represent modification sites. More detailed evidence for these modification sites is presented in the Post-translational Modifications table, located just below the protein sequence.


1 MSHHKKRVYP QAQVPYIASM PIVAEQQQSQ QQIDQTAYAM GNLQLNNRAN SFTQLAQNQQ
61 FPGSGKVVNQ LYPVDLFTEL PPPIRDLSLP PLPITISQDN IVTPSEYSNV PYQYVRSTLK
121 AVPKTNSLLK KTKLPFAIVI RPYLHLQDSD NQVPLNTDGV IVRCRRCRSY MNPFVVFINQ
181 GRKWQCNICR FKNDVPFGFD QNLQGAPINR YERNEIKNSV VDYLAPVEYS VREPPPSVYV
241 FLLDVSQNAV KNGLLATSAR TILENIEFLP NHDGRTRIAI ICVDHSLHYF YVPLDDDYEV
301 SDEDDEESDG EEEDEDEEEE DVDNSETIQM FDIGDLDEPF LPMPSDELVV PLKYCKNNLE
361 TLLKKIPEIF QDTHSSKFAL GPALKAASNL IKSTGGKVEV ISSTLPNTGI GKLKKRSEQG
421 ILNTPKESSQ LLSCKDSFYK TFTIECNKLQ ITVDMFLASE DYMDVATLSH LGRFSGGQTH
481 FYPGFNATSL NDVTKFTREL SRHLSMDISM EAVMRVRCST GLRATSFFGH FFNRSSDLCA
541 FSTMPRDQSY LFGISIEDSL MAEYCYLQVS TLLTLNTGER RIRVMTLALP TSESAREVFA
601 SADQLAITDF MTQNAVTKAL NSSMYSARDF ITKSLEDILN AYKKEISMSN INSVTSLNLC
661 ANLRMLPLLM NGLSKHIALR PGVVPSDYRA SALNRLETEP LHYLIKSIYP TVYSLHDMPD
721 EVGLPDFEGK TVLPEPINAT ISLFERYGLY LIDNSAELFL WVGGDAVPEL LIDVFNTDTI
781 SQIPVGKSEL PLLNDSPFNE RLRRIIGRIR ENNDTITFQS LYIIRGPSIN EPANLNSEKD
841 MASLRLWVLS TLVEDKVLNC ASYREYLQSM KTSINR*

* Blue amino acids indicate modification sites. More information below.

Post-translational Modifications - S288C

Modification sites for the protein in the selected strain, based on the presence of a residue in the specific strain, as inferred from experimental evidence.

20 entries for 8 sites

Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through its pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

SiteModificationModifierReference
S51phosphorylated residueZhou X, et al. (2021) PMID: 33481703
S51phosphorylated residueAlbuquerque CP, et al. (2008) PMID: 18407956
S51phosphorylated residueHolt LJ, et al. (2009) PMID: 19779198
S51phosphorylated residueMacGilvray ME, et al. (2020) PMID: 32597660
S51phosphorylated residueLanz MC, et al. (2021) PMID: 33491328
S51phosphorylated residueSoulard A, et al. (2010) PMID: 20702584
S51phosphorylated residueSwaney DL, et al. (2013) PMID: 23749301
S51phosphorylated residueLeutert M, et al. (2023) PMID: 37845410
T53phosphorylated residueZhou X, et al. (2021) PMID: 33481703
T53phosphorylated residueAlbuquerque CP, et al. (2008) PMID: 18407956
Showing 1 to 10 of 20 entries

Sequence-Based Physico-chemical Properties - S288C

Calculated protein properties, including amino acid composition, length, coding region calculations, and atomic composition.

Amino Acid Composition

Sort table using the arrow to the right of a column header to sort by that column; download all properties as a .txt file using the "Download Properties" button.

Amino AcidFrequencyPercentage
A455.14
C131.48
D546.16
E556.28
F384.34
G354.00
H141.60
I556.28
K394.45
L9610.96
M222.51
N556.28
P525.94
Q374.22
R455.14
S799.02
T505.71
V556.28
W30.34
Y343.88

Physical Details

Length (a.a): 876
Molecular Weight (Da): 98919.4
Isoelectric Point (pl): 4.95
Formula: C4397H6900N1173O1350S35
Aliphatic Index: 86.58
Instability Index: 50.12

Coding Region Translation Calculations

Codon Bias: 0.1
Codon Adaptation Index: 0.15
Frequence of Optimal Codons: 0.48
Hydropathicity of Protein: -0.27
Aromaticity Score: 0.09

Extinction Coefficients at 280nm

ALL Cys residues appear as half cystines: 67910.0
NO Cys residues appear as half cystines: 67160.0

Atomic Composition

Sort table using the arrow to the right of a column header to sort by that column; download all properties as a .txt file using the "Download Properties" button.

Atom Frequency Percentage

Data not found or not available for  S288C

External Identifiers

List of external identifiers for the protein from various database sources.

29 entries for 7 sources


Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through its pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

External IDSource
4370DIP
U12141GenBank/EMBL/DDBJ
6324279GenBank/EMBL/DDBJ
1730731GenBank/EMBL/DDBJ
1149539GenBank/EMBL/DDBJ
51013107GenBank/EMBL/DDBJ
1098489GenBank/EMBL/DDBJ
3355837GenBank/EMBL/DDBJ
2253169GenBank/EMBL/DDBJ
1301909GenBank/EMBL/DDBJ
Showing 1 to 10 of 29 entries

Resources