Phenotype Help

ASI3 / YNL008C Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided.


Summary
Non-essential gene; overexpression causes an increase in chromosome instability; in systematic studies null mutation confers resistance to selenomethionine and tirapazamine, increased competitive fitness, and shortened chronological lifespan

Annotations

A phenotype is defined as an observable (e.g., apoptosis) and a qualifier (e.g., increased). There may be more than one row with the same phenotype if that phenotype was observed in separate studies or in different conditions, strains, alleles, etc.

11 entries for 9 phenotypes


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.

PhenotypeExperiment TypeMutant InformationStrain BackgroundChemicalDetailsReference
chronological lifespan: decreased
competitive growth

competitive survival of mixed stationary phase co-cultures

null
Allele: asi3-Δ
S288CGaray E, et al. (2014) PMID:24586198
colony sectoring: increased
classical geneticsoverexpressionS288CAssay: quantitative chromosome transmission fidelity (qCTF) assay
Details: >1.5 fold increase in mini-chromosome loss relative to wt, indicative of chromosome instability (CIN)
Zhu J, et al. (2015) PMID:25823586
competitive fitness: increased
competitive growth

fitness profiling using complete deletion alleles

null
Allele: asi3-Δ
S288CMedia: minimal medium
Details: Relative fitness score: 1.001
Breslow DK, et al. (2008) PMID:18622397
killer toxin resistance: decreased
systematic mutation setnull
Allele: asi3-Δ
S288CDetails: K28 killer toxin
Carroll SY, et al. (2009) PMID:19853568
resistance to chemicals: decreased
classical genetics null
Allele: asi3-Δ
S288C75 μg/ml hygromycin BWoodruff KA, et al. (2021) PMID:34095778
resistance to chemicals: decreased
systematic mutation set null
Allele: asi3-Δ
S288C60 mM 5-hydroxymethylfurfuralLi Q, et al. (2023) PMID:38049732
resistance to chemicals: increased
systematic mutation setnull
Allele: asi3-Δ
S288C0.2 mM selenomethionineBockhorn J, et al. (2008) PMID:19004804
resistance to chemicals: increased
systematic mutation setnull
Allele: asi3-Δ
S288C500 uM tirapazamineHellauer K, et al. (2005) PMID:16061773
starvation resistance: decreased
heterozygous diploid, competitive growth

measurement of fitness in a fluctuating environment, modeled using a semi-batch fermentor system

null
Allele: asi3-Δ
S288CDavey HM, et al. (2012) PMID:22356628
vacuolar morphology: abnormal
systematic mutation set

screen all non-essential genes for inability to fragment vacuoles in response to salt addition

null
Allele: asi3-Δ
S288C0.4 M sodium chlorideDetails: moderate defect in vacuolar fragmentation
Michaillat L and Mayer A (2013) PMID:23383298
Showing 1 to 10 of 11 entries

Shared Phenotypes

This diagram displays phenotype observables (purple squares) that are shared between the given gene (yellow circle) and other genes (gray circles) based on the number of phenotype observables shared (adjustable using the slider at the bottom).


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Click on a gene or phenotype observable name to go to its specific page within SGD; drag any of the gene or observable objects around within the visualization for easier viewing; click “Reset” to automatically redraw the diagram; filter the genes that share observable terms with the given gene by the number of terms they share by clicking anywhere on the slider bar or dragging the tab to the desired filter number.


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