TRM9 / YML014W Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided.

Summary

TRM9/YML014W is a non-essential gene in reference strain S288C, null mutants are viable, haploinsufficient, and grow slowly, with reduced competitive and fermentative performance and increased heat sensitivity. They show enlarged cells with abnormal buds, vacuolar defects (especially in high salt), diminished filamentous/invasive growth, altered colony appearance, and reduced endocytosis and protein secretion. RNA/tRNA modification and protein accumulation are reduced, amino‑acid balance is perturbed (global α‑amino‑acid imbalance with increased arginine and decreased proline), gadolinium accumulates, and strains are myo‑inositol auxotrophs. Stress phenotypes include increased oxidative stress tolerance (paraquat, H2O2) despite aminoglycoside‑induced ROS, decreased desiccation resistance, shorter replicative lifespan, and context‑dependent respiration (enhanced on nonfermentable carbon at ambient but impaired on ethanol at 37°C); resistance to visible light is reduced. Drug sensitivity is broad: mutants are hypersensitive to aminoglycosides and hygromycin, DNA damage/replication inhibitors (MMS, hydroxyurea, bleomycin), cell wall/membrane agents (Calcofluor White, Congo Red, caspofungin, miconazole), HDAC inhibitors, alcohols/solvents, arsenite, aflatoxin B1, sirolimus, and metals (Zn, Hg, Gd), while resistance is increased to actinomycin D, benomyl, tirapazamine, and certain toxins; wortmannin sensitivity is normal. Biofilm formation is increased, and tolerance to low zinc is improved.

Annotations

A phenotype is defined as an observable (e.g., apoptosis) and a qualifier (e.g., increased). There may be more than one row with the same phenotype if that phenotype was observed in separate studies or in different conditions, strains, alleles, etc.

Shared Phenotypes

This diagram displays phenotype observables (purple squares) that are shared between the given gene (yellow circle) and other genes (gray circles) based on the number of phenotype observables shared (adjustable using the slider at the bottom).

Resources