Protein Help

AFG2 / YLR397C Protein

Protein abundance data, domains, shared domains with other proteins, protein sequence retrieval for various strains, sequence-based physico-chemical properties, protein modification sites, and external identifiers for the protein.


Aliases
DRG1 6
Protein Product
AAA family ATPase AFG2
Feature Type
ORF , Verified
EC Number
3.6.4.10

AlphaFold Protein Structure

AlphaFold, developed by DeepMind, is an AI program that accurately predicts protein structures from amino acid sequences, enabling visualization of protein conformations. The predicted structures can be accessed through the Protein Data Bank (PDB) and AlphaFold Protein Structure Database.


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Model Confidence

Very high
Confident
Low
Very low

Experimental Data

Contains experimentally-derived protein half-life data obtained using stable isotope labeling by amino acids (SILAC) coupled with mass spectrometry. This section also contains protein abundance data for both untreated and treated cells obtained from over 20 studies. These data have been normalized and converted to a common unit of molecules per cell.


Protein Half Life

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ExperimentResultReference
half-life9.8 hrChristiano R, et al. (2014)

Protein Abundance

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Abundance (molecules/cell)MediaTreatmentTreatment timeFold ChangeVisualizationStrain backgroundOriginal ReferenceReference
2949SDuntreatedconfocal microscopy evidenceS288CBreker M, et al. (2013)Ho B, et al. (2018)
1788SD2 mM 1,4-dithiothreitol2 hrconfocal microscopy evidenceS288CBreker M, et al. (2013)Ho B, et al. (2018)
2393SD1 mM hydrogen peroxide1 hrconfocal microscopy evidenceS288CBreker M, et al. (2013)Ho B, et al. (2018)
1961SD minus nitrogencellular response to nitrogen starvation15 hrconfocal microscopy evidenceS288CBreker M, et al. (2013)Ho B, et al. (2018)
2743SDuntreatedconfocal microscopy evidenceS288CChong YT, et al. (2015)Ho B, et al. (2018)
Showing 1 to 5 of 23 entries

Domains and Classification - S288C

Collection of computationally identified domains and motifs, as determined by InterProScan analysis; includes protein coordinates for the domain, a domain Description, a Source and corresponding accession ID, and the number of S. cerevisiae genes that share the same domain.


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Gene Protein Coordinates Accession ID Description Source No. of Genes with Domain

Domain Locations

Visual representation of the locations of the domains within the protein, as listed in the Domains and Classification table. Each row displays the domain(s) derived from a different Source, with domains color-coded according to this Source.

Scroll over a domain to view its exact coordinates and its Description.

Shared Domains

This diagram displays domains (colored squares) shared between the given protein (yellow circle) and other proteins (gray circles); the domains are color-coded according to their source, as displayed in the Domain Locations table, above.

Reset

Click on a gene or domain name to go to its specific page within SGD; drag any of the gene or domain objects around within the visualization for easier viewing; click “Reset” to automatically redraw the diagram.

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.


afg2-(drg1-1) | afg2-(drg1-18) | afg2-(drg1-I692T) | afg2-(drg1-L555F) | afg2-(drg1-V656A) | afg2-(drg1-V725A) | afg2-(drg1-V725E) | ... Show all

View all AFG2 alleles in SGD search

Sequence

Protein sequence for the given gene in S288C and other strains, when available. Use the pull-down menu under "Strain" to select the sequence for a specific strain. The displayed sequence can be downloaded in FASTA format as a .txt file. Amino acids displayed in blue represent modification sites. More detailed evidence for these modification sites is presented in the Post-translational Modifications table, located just below the protein sequence.


1 MAPKSSSSGS KKKSSASSNS ADAKASKFKL PAEFITRPHP SKDHGKETCT AYIHPNVLSS
61 LEINPGSFCT VGKIGENGIL VIARAGDEEV HPVNVITLST TIRSVGNLIL GDRLELKKAQ
121 VQPPYATKVT VGSLQGYNIL ECMEEKVIQK LLDDSGVIMP GMIFQNLKTK AGDESIDVVI
181 TDASDDSLPD VSQLDLNMDD MYGGLDNLFY LSPPFIFRKG STHITFSKET QANRKYNLPE
241 PLSYAAVGGL DKEIESLKSA IEIPLHQPTL FSSFGVSPPR GILLHGPPGT GKTMLLRVVA
301 NTSNAHVLTI NGPSIVSKYL GETEAALRDI FNEARKYQPS IIFIDEIDSI APNRANDDSG
361 EVESRVVATL LTLMDGMGAA GKVVVIAATN RPNSVDPALR RPGRFDQEVE IGIPDVDARF
421 DILTKQFSRM SSDRHVLDSE AIKYIASKTH GYVGADLTAL CRESVMKTIQ RGLGTDANID
481 KFSLKVTLKD VESAMVDIRP SAMREIFLEM PKVYWSDIGG QEELKTKMKE MIQLPLEASE
541 TFARLGISAP KGVLLYGPPG CSKTLTAKAL ATESGINFLA VKGPEIFNKY VGESERAIRE
601 IFRKARSAAP SIIFFDEIDA LSPDRDGSST SAANHVLTSL LNEIDGVEEL KGVVIVAATN
661 RPDEIDAALL RPGRLDRHIY VGPPDVNARL EILKKCTKKF NTEESGVDLH ELADRTEGYS
721 GAEVVLLCQE AGLAAIMEDL DVAKVELRHF EKAFKGIARG ITPEMLSYYE EFALRSGSSS
781 *

* Blue amino acids indicate modification sites. More information below.

Post-translational Modifications - S288C

Modification sites for the protein in the selected strain, based on the presence of a residue in the specific strain, as inferred from experimental evidence.

4 entries for 4 sites

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SiteModificationModifierReference
S221phosphorylated residueLeutert M, et al. (2023) PMID: 37845410
S277phosphorylated residueLanz MC, et al. (2021) PMID: 33491328
Y768phosphorylated residueLeutert M, et al. (2023) PMID: 37845410
Y769phosphorylated residueLeutert M, et al. (2023) PMID: 37845410
Showing 1 to 4 of 4 entries

Sequence-Based Physico-chemical Properties - S288C

Calculated protein properties, including amino acid composition, length, coding region calculations, and atomic composition.

Amino Acid Composition

Sort table using the arrow to the right of a column header to sort by that column; download all properties as a .txt file using the "Download Properties" button.

Amino AcidFrequencyPercentage
A668.46
C70.90
D496.28
E577.31
F273.46
G577.31
H141.79
I577.31
K516.54
L739.36
M182.31
N283.59
P425.38
Q151.92
R384.87
S698.85
T415.26
V526.67
W10.13
Y182.31

Physical Details

Length (a.a): 780
Molecular Weight (Da): 84731.5
Isoelectric Point (pl): 5.42
Formula: C3746H6020N1017O1164S25
Aliphatic Index: 90.89
Instability Index: 40.59

Coding Region Translation Calculations

Codon Bias: 0.06
Codon Adaptation Index: 0.13
Frequence of Optimal Codons: 0.46
Hydropathicity of Protein: -0.17
Aromaticity Score: 0.06

Extinction Coefficients at 280nm

ALL Cys residues appear as half cystines: 32695.0
NO Cys residues appear as half cystines: 32320.0

Atomic Composition

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Atom Frequency Percentage

Data not found or not available for  S288C

External Identifiers

List of external identifiers for the protein from various database sources.

23 entries for 10 sources


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External IDSource
orf19.6432CGD
4470DIP
3.6.4.10ExPASy
AY693116GenBank/EMBL/DDBJ
L14615GenBank/EMBL/DDBJ
6323429GenBank/EMBL/DDBJ
U19729GenBank/EMBL/DDBJ
AAC37367.1GenBank/EMBL/DDBJ
AAT93135.1GenBank/EMBL/DDBJ
AAB82355.1GenBank/EMBL/DDBJ
Showing 1 to 10 of 23 entries

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