Phenotype Help

CHS5 / YLR330W Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided.


Summary
Non-essential gene in reference strain S288C; null mutant has strong defect in delivering chitin synthase to plasma membrane that leads to decreased chitin content in cell wall; null mutant displays reduced mating, increased heat sensitivity and resistance to Calcofluor White; in systematic studies null mutants show elevated sensitivity to multiple chemicals, including rapamycin, wortmannin, caffeine, bleomycin, but increased resistance to tunicamycin and fenpropimorph

Annotations

A phenotype is defined as an observable (e.g., apoptosis) and a qualifier (e.g., increased). There may be more than one row with the same phenotype if that phenotype was observed in separate studies or in different conditions, strains, alleles, etc.

130 entries for 31 phenotypes


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PhenotypeExperiment TypeMutant InformationStrain BackgroundChemicalDetailsReference
axial budding pattern: decreased
homozygous diploidnull
Allele: chs5-Δ
S288CDetails: null mutant buds randomly, rather than in a bipolar pattern, as observed in wt diploids
Trautwein M, et al. (2006) PMID:16498409
cell fusion: decreased
classical geneticsnull
Allele: chs5-Δ
OtherTreatment: mated to wild type cells
Details: defective in cell fusion with increased prezygote formation, in which cell walls fuse but membranes do not
Dorer R, et al. (1997) PMID:9135999
chemical compound accumulation: decreased
homozygous diploidnull
Allele: chs5-Δ
S288C tellurium atom, 0.1 mM potassium telluriteOttosson LG, et al. (2010) PMID:20675578
chemical compound accumulation: decreased
homozygous diploid, systematic mutation setnull
Allele: chs5-Δ
S288C rubidium(1+)Details: Rb+ influx is a measure of K+ influx
Fell GL, et al. (2011) PMID:22384317
chemical compound excretion: increased
homozygous diploid, systematic mutation set

quantification of extracellular glutathione at stationary phase

null
Allele: chs5-Δ
S288C glutathioneTreatment: SD medium
Details: Fold elevation of glutathione excretion: 5
Perrone GG, et al. (2005) PMID:15509654
chitin deposition: decreased
systematic mutation setnull
Allele: chs5-Δ
S288CDetails: fluorescent Calcofluor white binding
Lam KK, et al. (2006) PMID:16818716
chitin deposition: decreased
classical geneticsnull
Allele: chs5-Δ
OtherDetails: chitin content is 25% of wild-type
Santos B, et al. (1997) PMID:9111317
chitin deposition: decreased
classical geneticsnull
Allele: chs5-Δ
S288CDetails: decreased calcofluor white staining of the cell wall
Trautwein M, et al. (2006) PMID:16498409
chitin deposition: decreased
systematic mutation setnull
Allele: chs5-Δ
S288CDetails: Chitin level (nmole GlcNAc/mg dry weight): 3.2
Lesage G, et al. (2005) PMID:15715908
competitive fitness: decreased
systematic mutation set

fitness profiling after 20 generations

null
Allele: chs5-Δ
S288C1 M sodium chlorideGiaever G, et al. (2002) PMID:12140549
Showing 1 to 10 of 130 entries

Shared Phenotypes

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