RCK2 / YLR248W Overview


Standard Name
RCK2 1
Systematic Name
YLR248W
SGD ID
SGD:S000004238
Aliases
CLK1 5 , CMK3 7
Feature Type
ORF , Verified
Description
Protein kinase involved in response to oxidative and osmotic stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; similar to CaM (calmodulin) kinases; RCK2 has a paralog, RCK1, that arose from the whole genome duplication 2 3 4 5 6
Name Description
Radiation sensitivity Complementing Kinase 1
Paralog
RCK1 6
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Summary
RCK2 is located on the right arm of chromosome XII between IRC20 E3 ubiquitin ligase and YEF3 translation elongation factor 3; coding sequence is 1833 nucleotides long with 22 SNPs, 9 of which cause amino acid polymorphisms; RCK2 has paralog RCK1 from the whole genome duplication
Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Summary
Rck2p is 610 amino acids long, of average half-life, low in abundance; succinylated on K193, ubiquitinylated on K38 and K431, phosphorylated on 19 residues
Length (a.a.)
610
Mol. Weight (Da)
68043.1
Isoelectric Point
5.1
Median Abundance (molecules/cell)
5272 +/- 2285
Half-life (hr)
9.4

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.


View all RCK2 alleles in SGD search

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
Serine/threonine kinase involved in osmosensory signaling and cellular response to oxidative stress; localized to cytoplasm

View computational annotations

Molecular Function

Manually Curated

Biological Process

Manually Curated

Cellular Component

Manually Curated
Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
Non-essential gene; null displays increased competitive fitness, is sensitive to salt, metals, antifungals, rapamycin; overexpression slows growth
Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


Summary
Rck2p interacts physically with proteins involved in cytoskeleton organization, RCK2 interacts genetically with genes involved in transcription; the rck2 null mutant is viable, the null mutant of paralog rck1 is viable, the rck2 rck1 double mutant has not been annotated for phenotype

178 total interactions for 141 unique genes

Physical Interactions

  • Affinity Capture-MS: 59
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 8
  • Biochemical Activity: 29
  • Co-localization: 2
  • PCA: 4
  • Reconstituted Complex: 5
  • Two-hybrid: 5

Genetic Interactions

  • Dosage Lethality: 3
  • Dosage Rescue: 9
  • Negative Genetic: 32
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 1
  • Positive Genetic: 7
  • Synthetic Growth Defect: 3
  • Synthetic Haploinsufficiency: 2
  • Synthetic Lethality: 1
  • Synthetic Rescue: 3
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Summary
RCK2 promoter is bound by Msn2p, Rfx1p, Tup1p in response to heat; Rck2 protein activity is regulated by Cdc28p; Rck2p protein activity is downregulated by protein phosphatase PP2A during hyperosmotic stress signaling
Regulators
5
Targets
0
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
28
Additional
41
Reviews
17

Resources