Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided.
A phenotype is defined as an observable (e.g., apoptosis) and a qualifier (e.g., increased). There may be more than one row with the same phenotype if that phenotype was observed in separate studies or in different conditions, strains, alleles, etc.
35 entries for 17 phenotypesIncrease the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.
| Phenotype | Experiment Type | Mutant Information | Strain Background | Chemical | Details | Reference |
|---|---|---|---|---|---|---|
| cell cycle progression in M phase: arrested | classical genetics | repressible | Other | Details: arrest with 2N DNA content, large bud, and single nucleus | Sobel SG and Snyder M (1995) PMID:8557744 | |
| cell cycle progression in M phase: arrested | classical genetics | conditional Allele: tub4-1 F244S (reported as F243S); >80% of cells complete S phase and arrest with a single large bud; number declines to 70% after 6 h, indicating that some cells overcome the cell cycle arrest | S288C | Temperature: elevated temperature, 37 °C | Spang A, et al. (1996) PMID:8707827 | |
| cell size: increased | heterozygous diploid, systematic mutation set analysis of cell size for mutants in the systematic deletion collection | null Allele: tub4-Δ | S288C | Details: mutant is among the largest 5% of heterozygous diploid deletion strains | Jorgensen P, et al. (2002) PMID:12089449 | |
| chromosome/plasmid maintenance: abnormal | classical genetics | conditional Allele: tub4-1 F244S (reported as F243S); >80% of cells complete S phase and arrest with a single large bud; number declines to 70% after 6 h, indicating that some cells overcome the cell cycle arrest | S288C | Temperature: permissive temperature, 30 °C Details: increased chromosome loss in 50% of cells at 30 deg C and 74% of cells at 33 deg C | Spang A, et al. (1996) PMID:8707827 | |
| chromosome/plasmid maintenance: decreased | heterozygous diploid | null Allele: tub4-Δ | S288C | Details: haploinsufficiency results in an increased frequency of chromosome loss, indicative of chromosome instability (CIN) | Choy JS, et al. (2013) PMID:23825022 | |
| chromosome/plasmid maintenance: decreased | heterozygous diploid, systematic mutation set | null Allele: tub4-Δ | S288C | Assay: diploid bimater (BiM) assay Details: haploinsufficiency results in chromosome instability (CIN); scored >= 2 standard deviations above the mean | Choy JS, et al. (2013) PMID:23825022 | |
| colony sectoring: increased | large-scale survey | conditional Allele: tub4-Y445D | S288C | Assay: chromosome transmission fidelity (CTF) assay Temperature: semi-permissive temperature Details: indicative of chromosome instability (CIN); metascore strong (multiple hit); similar results with tub4-deltaDSY | Stirling PC, et al. (2011) PMID:21552543 | |
| competitive fitness: decreased | competitive growth fitness profiling of essential genes using hypomorphic DAmP alleles | reduction of function | S288C | Media: minimal medium Details: Relative fitness score: 0.987 | Breslow DK, et al. (2008) PMID:18622397 | |
| haploinsufficient | heterozygous diploid, competitive growth genome-wide fitness profiling | null Allele: tub4-Δ | S288C | Media: turbidostat growth in FPM medium Details: Relative growth score: -0.0031 | Pir P, et al. (2012) PMID:22244311 | |
| heat sensitivity: increased | classical genetics | conditional Allele: tub4-S360D phosphomimetic mutation in a surface loop that may participate in protein-protein interactions | W303 | Temperature: elevated temperature, 37 °C | Keck JM, et al. (2011) PMID:21700874 |
This diagram displays phenotype observables (purple squares) that are shared between the given gene (yellow circle) and other genes (gray circles) based on the number of phenotype observables shared (adjustable using the slider at the bottom).
Click on a gene or phenotype observable name to go to its specific page within SGD; drag any of the gene or observable objects around within the visualization for easier viewing; click “Reset” to automatically redraw the diagram; filter the genes that share observable terms with the given gene by the number of terms they share by clicking anywhere on the slider bar or dragging the tab to the desired filter number.
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