Phenotype Help

PNP1 / YLR209C Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided.


Summary
Non-essential gene; null mutant excretes guanosine and inosine, and shows decreased autophagy under nitrogen starvation; in systematic studies null mutant displays increased sensitivity to oxidative stress and miconazole, and decreased glycogen accumulation

Annotations

A phenotype is defined as an observable (e.g., apoptosis) and a qualifier (e.g., increased). There may be more than one row with the same phenotype if that phenotype was observed in separate studies or in different conditions, strains, alleles, etc.

12 entries for 9 phenotypes


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.

PhenotypeExperiment TypeMutant InformationStrain BackgroundChemicalDetailsReference
autophagy: decreased
classical genetics

autophagy measured using Pho8delta60 assay

null
Allele: pnp1-Δ
S288CMedia: nitrogen starvation
Dengjel J, et al. (2012) PMID:22311637
chemical compound accumulation: decreased
homozygous diploid, systematic mutation set

glycogen accumulation in diploid mutant strains grown in microtiter plates

null
Allele: pnp1-Δ
S288C glycogenWilson WA, et al. (2002) PMID:12096123
chemical compound excretion: increased
classical geneticsnull
Allele: pnp1-Δ
S288C inosineLecoq K, et al. (2001) PMID:11466296
chemical compound excretion: increased
classical geneticsnull
Allele: pnp1-Δ
S288C guanosineLecoq K, et al. (2001) PMID:11466296
chronological lifespan: decreased
competitive growth

competitive survival of mixed stationary phase co-cultures

null
Allele: pnp1-Δ
S288CGaray E, et al. (2014) PMID:24586198
competitive fitness: normal
competitive growth

fitness profiling using complete deletion alleles

null
Allele: pnp1-Δ
S288CMedia: minimal medium
Details: Relative fitness score: 1
Breslow DK, et al. (2008) PMID:18622397
haploinsufficient
heterozygous diploid, competitive growth

genome-wide fitness profiling

null
Allele: pnp1-Δ
S288CMedia: turbidostat growth in FPM medium
Details: Relative growth score: -0.0054
Pir P, et al. (2012) PMID:22244311
oxidative stress resistance: decreased
homozygous diploid, competitive growthnull
Allele: pnp1-Δ
S288C3 mM hydrogen peroxideBrown JA, et al. (2006) PMID:16738548
resistance to chemicals: decreased
systematic mutation setnull
Allele: pnp1-Δ
S288C1000 ug/ml miconazoleVandenbosch D, et al. (2013) PMID:24034557
resistance to chemicals: decreased
systematic mutation setnull
Allele: pnp1-Δ
S288C1 mM 4-chlorophenolYadav V, et al. (2011) PMID:20967895
Showing 1 to 10 of 12 entries

Shared Phenotypes

This diagram displays phenotype observables (purple squares) that are shared between the given gene (yellow circle) and other genes (gray circles) based on the number of phenotype observables shared (adjustable using the slider at the bottom).


Reset

Click on a gene or phenotype observable name to go to its specific page within SGD; drag any of the gene or observable objects around within the visualization for easier viewing; click “Reset” to automatically redraw the diagram; filter the genes that share observable terms with the given gene by the number of terms they share by clicking anywhere on the slider bar or dragging the tab to the desired filter number.


Resources