Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided.
A phenotype is defined as an observable (e.g., apoptosis) and a qualifier (e.g., increased). There may be more than one row with the same phenotype if that phenotype was observed in separate studies or in different conditions, strains, alleles, etc.
17 entries for 11 phenotypesIncrease the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.
| Phenotype | Experiment Type | Mutant Information | Strain Background | Chemical | Details | Reference |
|---|---|---|---|---|---|---|
| autophagy: decreased duration | classical genetics | null Allele: tag1-Δ461-608 C-terminal truncation, AA 461-608 deleted; partial deletion of the luminal region | Other | Media: nitrogen starvation Details: alkaline phosphatase (ALP) activity assayed using cytosolic Pho8Δ60 is increased like wt but exhibited no decrease after 8 hrs with higher activity at 24 hrs | Kira S, et al. (2021) PMID:33536246 | |
| autophagy: decreased duration | classical genetics | null Allele: tag1-Δ113-608 C-terminal truncation, AA 113-608 deleted; partial deletion of the luminal region | Other | Media: nitrogen starvation Details: alkaline phosphatase (ALP) activity assayed using cytosolic Pho8Δ60 is increased like wt but exhibited no decrease after 8 hrs with higher activity at 24 hrs | Kira S, et al. (2021) PMID:33536246 | |
| autophagy: decreased duration | classical genetics | null Allele: tag1-Δ81-608 C-terminal truncation, AA 81-608 deleted; deletion of the luminal region | Other | Media: nitrogen starvation Details: alkaline phosphatase (ALP) activity assayed using cytosolic Pho8Δ60 is increased like wt but exhibited no decrease after 8 hrs with higher activity at 24 hrs | Kira S, et al. (2021) PMID:33536246 | |
| autophagy: decreased duration | classical genetics | null Allele: tag1-Δ113-264 deletion of AA 113-264; partial deletion of the luminal region | Other | Media: nitrogen starvation Details: alkaline phosphatase (ALP) activity assayed using cytosolic Pho8Δ60 is increased like wt but exhibited no decrease after 8 hrs with higher activity at 24 hrs | Kira S, et al. (2021) PMID:33536246 | |
| autophagy: increased duration | classical genetics | null Allele: tag1-Δ | S288C | Media: nitrogen starvation Details: alkaline phosphatase (ALP) activity assayed using cytosolic Pho8Δ60 is increased like wt until 8 hrs, but exhibited no decrease after 8 hrs and had 1.9-fold higher activity at 24 hrs; autophagic bodies also accumulate | Kira S, et al. (2021) PMID:33536246 | |
| competitive fitness: decreased | competitive growth | overexpression | S288C | Details: decreased abundance within the barFLEX overexpression collection arrayed after 20 generations of pooled growth | Douglas AC, et al. (2012) PMID:23050238 | |
| competitive fitness: increased | competitive growth fitness profiling using complete deletion alleles | null Allele: tag1-Δ | S288C | Media: minimal medium Details: Relative fitness score: 1.005 | Breslow DK, et al. (2008) PMID:18622397 | |
| competitive fitness: increased | systematic mutation set | null Allele: tag1-Δ | S288C | Media: rich medium (YPD) w/6% ethanol, ETH | Qian W, et al. (2012) PMID:23103169 | |
| protein/peptide distribution: abnormal Reporter: Atg13-GFP | classical genetics | null Allele: tag1-Δ | S288C | Media: nitrogen starvation Details: increased Atg13p perivacuolar puncta, representing the PAS, present after 24 hr of nitrogen starvation, relative to wt where Atg13 puncta are induced early but decreased after prolonged starvation and PAS disassembly | Kira S, et al. (2021) PMID:33536246 | |
| protein/peptide distribution: abnormal Reporter: Atg2-GFP | classical genetics | null Allele: tag1-Δ | S288C | Media: nitrogen starvation Details: increased Atg2p perivacuolar puncta, representing the PAS, present after 24 hr of nitrogen starvation, relative to wt where Atg2 puncta are induced early but decreased after prolonged starvation and PAS disassembly | Kira S, et al. (2021) PMID:33536246 |
This diagram displays phenotype observables (purple squares) that are shared between the given gene (yellow circle) and other genes (gray circles) based on the number of phenotype observables shared (adjustable using the slider at the bottom).
Click on a gene or phenotype observable name to go to its specific page within SGD; drag any of the gene or observable objects around within the visualization for easier viewing; click “Reset” to automatically redraw the diagram; filter the genes that share observable terms with the given gene by the number of terms they share by clicking anywhere on the slider bar or dragging the tab to the desired filter number.
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