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TIS11 / YLR136C Protein

Protein abundance data, domains, shared domains with other proteins, protein sequence retrieval for various strains, sequence-based physico-chemical properties, protein modification sites, and external identifiers for the protein.

Aliases
CTH2 2
Feature Type
ORF , Verified
Paralog
CTH1 5
Summary
Tis11p is 285 amino acids long and low in abundance; relative distribution to the nucleus increases upon DNA replication stress; has 2 zinc-finger domains; phosphorylated on 5 serines

AlphaFold Protein Structure

AlphaFold, developed by DeepMind, is an AI program that accurately predicts protein structures from amino acid sequences, enabling visualization of protein conformations. The predicted structures can be accessed through the Protein Data Bank (PDB) and AlphaFold Protein Structure Database.

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Model Confidence

Very high
Confident
Low
Very low

Experimental Data

Contains experimentally-derived protein half-life data obtained using stable isotope labeling by amino acids (SILAC) coupled with mass spectrometry. This section also contains protein abundance data for both untreated and treated cells obtained from over 20 studies. These data have been normalized and converted to a common unit of molecules per cell.

Protein Half Life

No half-life data available for TIS11.

Protein Abundance

Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through its pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

Abundance (molecules/cell)MediaTreatmentTreatment timeFold ChangeVisualizationStrain backgroundOriginal ReferenceReference
2074SDuntreatedconfocal microscopy evidenceS288CBreker M, et al. (2013)Ho B, et al. (2018)
2467SD2 mM 1,4-dithiothreitol2 hrconfocal microscopy evidenceS288CBreker M, et al. (2013)Ho B, et al. (2018)
4323SD1 mM hydrogen peroxide1 hrconfocal microscopy evidenceS288CBreker M, et al. (2013)Ho B, et al. (2018)
1375SD minus nitrogencellular response to nitrogen starvation15 hrconfocal microscopy evidenceS288CBreker M, et al. (2013)Ho B, et al. (2018)
4346SD0.2 M hydroxyurea160 minconfocal microscopy evidenceS288CChong YT, et al. (2015)Ho B, et al. (2018)
Showing 1 to 5 of 8 entries

Domains and Classification - S288C

Collection of computationally identified domains and motifs, as determined by InterProScan analysis; includes protein coordinates for the domain, a domain Description, a Source and corresponding accession ID, and the number of S. cerevisiae genes that share the same domain.

Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through its pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

Gene Protein Coordinates Accession ID Description Source No. of Genes with Domain

Domain Locations

Visual representation of the locations of the domains within the protein, as listed in the Domains and Classification table. Each row displays the domain(s) derived from a different Source, with domains color-coded according to this Source.

Scroll over a domain to view its exact coordinates and its Description.

Shared Domains

This diagram displays domains (colored squares) shared between the given protein (yellow circle) and other proteins (gray circles); the domains are color-coded according to their source, as displayed in the Domain Locations table, above.

Reset

Click on a gene or domain name to go to its specific page within SGD; drag any of the gene or domain objects around within the visualization for easier viewing; click “Reset” to automatically redraw the diagram.

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.

TIS11-S64A,S65A | tis11-C190R | tis11-S64A,S65A,C190R | tis11-Δ

View all TIS11 alleles in SGD search

Sequence

Protein sequence for the given gene in S288C and other strains, when available. Use the pull-down menu under "Strain" to select the sequence for a specific strain. The displayed sequence can be downloaded in FASTA format as a .txt file. Amino acids displayed in blue represent modification sites. More detailed evidence for these modification sites is presented in the Post-translational Modifications table, located just below the protein sequence. 

  1 MWAQLSYTRP ESQKTDLTSL FSTDQEQNPL NDYQYQINIR ELEEYYNKTI LNEDNIQETS 
 61 SEISSAVSFS PPKNTNAIQP GLLYDPQLMN PFLPSAHLNS TAPTTFKKKL EVQINPDYVP 
121 KSSQLPLTSQ NLQQLSQQKP KNDASFSSEK ESSAQPKVKS QVQETPKQLY KTELCESFTL 
181 KGSCPYGSKC QFAHGLGELK VKKSCKNFRT KPCVNWEKLG YCPYGRRCCF KHGDDNDIAV 
241 YVKAGTYCNV SSTSKQSDEK RSNGRGSAKK KNLNVKVKAL QRMTW*

* Blue amino acids indicate modification sites. More information below.

Post-translational Modifications - S288C

Modification sites for the protein in the selected strain, based on the presence of a residue in the specific strain, as inferred from experimental evidence.

5 entries for 5 sites

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SiteModificationModifierReference
S64phosphorylated residueHRR25Romero AM, et al. (2018) PMID: 30228242
S65phosphorylated residueHRR25Romero AM, et al. (2018) PMID: 30228242
S68phosphorylated residueRomero AM, et al. (2018) PMID: 30228242
S70phosphorylated residueRomero AM, et al. (2018) PMID: 30228242
S160phosphorylated residueZhou X, et al. (2021) PMID: 33481703
Showing 1 to 5 of 5 entries

Sequence-Based Physico-chemical Properties - S288C

Calculated protein properties, including amino acid composition, length, coding region calculations, and atomic composition.

Amino Acid Composition

Sort table using the arrow to the right of a column header to sort by that column; download all properties as a .txt file using the "Download Properties" button.

Amino AcidFrequencyPercentage
A124.21
C93.16
D113.86
E175.96
F93.16
G113.86
H31.05
I82.81
K3110.88
L248.42
M31.05
N207.02
P186.32
Q238.07
R82.81
S3211.23
T186.32
V124.21
W31.05
Y134.56

Physical Details

Length (a.a): 285
Molecular Weight (Da): 32325.9
Isoelectric Point (pl): 9.26
Formula: C1417H2245N392O448S12
Aliphatic Index: 57.0
Instability Index: 43.89

Coding Region Translation Calculations

Codon Bias: 0.07
Codon Adaptation Index: 0.14
Frequence of Optimal Codons: 0.47
Hydropathicity of Protein: -0.89
Aromaticity Score: 0.09

Extinction Coefficients at 280nm

ALL Cys residues appear as half cystines: 36370.0
NO Cys residues appear as half cystines: 35870.0

Atomic Composition

Sort table using the arrow to the right of a column header to sort by that column; download all properties as a .txt file using the "Download Properties" button.

Atom Frequency Percentage

Data not found or not available for  S288C

External Identifiers

List of external identifiers for the protein from various database sources.

31 entries for 9 sources

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External IDSource
orf19.5334CGD
5614DIP
S76619GenBank/EMBL/DDBJ
L42134GenBank/EMBL/DDBJ
AY558210GenBank/EMBL/DDBJ
Z73308GenBank/EMBL/DDBJ
CAA97707.1GenBank/EMBL/DDBJ
CAA62651.1GenBank/EMBL/DDBJ
AAB82400.1GenBank/EMBL/DDBJ
AAS56536.1GenBank/EMBL/DDBJ
Showing 1 to 10 of 31 entries

Resources

Homologs

AGD | AnalogYeast | BLASTP at NCBI | CGD | FungiDB | PhylomeDB | PomBase | YGOB | YOGY

Protein Databases

AlphaFold Protein Structure | GPMDB | ModelArchive | Pfam domains | SUPERFAMILY | TopologYeast | UniProtKB

Localization

CYCLoPs | dHITS | LoQAtE | YeastGFP | YeastRC Public Images | YeastRGB | YPL+

Post-translational Modifications

CoSMoS.c. | PhosphoGRID | PhosphoPep