Protein Help

MET5 / YJR137C Protein

Protein abundance data, domains, shared domains with other proteins, protein sequence retrieval for various strains, sequence-based physico-chemical properties, protein modification sites, and external identifiers for the protein.


Aliases
ECM17 4
Protein Product
sulfite reductase (NADPH) subunit beta
Feature Type
ORF , Verified
EC Number
1.8.1.2
Summary
Met5p is 1442 amino acids long, of average half-life, low in abundance; contains 4 flavodoxin-like domains and 2 nitrite/sulphite reductase iron-sulphur/sirohaem-binding sites; phosphorylated on 12 residues

AlphaFold Protein Structure

AlphaFold, developed by DeepMind, is an AI program that accurately predicts protein structures from amino acid sequences, enabling visualization of protein conformations. The predicted structures can be accessed through the Protein Data Bank (PDB) and AlphaFold Protein Structure Database.


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Model Confidence

Very high
Confident
Low
Very low

Experimental Data

Contains experimentally-derived protein half-life data obtained using stable isotope labeling by amino acids (SILAC) coupled with mass spectrometry. This section also contains protein abundance data for both untreated and treated cells obtained from over 20 studies. These data have been normalized and converted to a common unit of molecules per cell.


Protein Half Life

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ExperimentResultReference
half-life10.3 hrChristiano R, et al. (2014)

Protein Abundance

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Abundance (molecules/cell)MediaTreatmentTreatment timeFold ChangeVisualizationStrain backgroundOriginal ReferenceReference
5289SDuntreatedconfocal microscopy evidenceS288CBreker M, et al. (2013)Ho B, et al. (2018)
6683SD2 mM 1,4-dithiothreitol2 hr1.26confocal microscopy evidenceS288CBreker M, et al. (2013)Ho B, et al. (2018)
9040SD1 mM hydrogen peroxide1 hr1.71confocal microscopy evidenceS288CBreker M, et al. (2013)Ho B, et al. (2018)
34717SD minus nitrogencellular response to nitrogen starvation15 hr6.56confocal microscopy evidenceS288CBreker M, et al. (2013)Ho B, et al. (2018)
2236SDuntreatedconfocal microscopy evidenceS288CChong YT, et al. (2015)Ho B, et al. (2018)
Showing 1 to 5 of 25 entries

Domains and Classification - S288C

Collection of computationally identified domains and motifs, as determined by InterProScan analysis; includes protein coordinates for the domain, a domain Description, a Source and corresponding accession ID, and the number of S. cerevisiae genes that share the same domain.


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Gene Protein Coordinates Accession ID Description Source No. of Genes with Domain

Domain Locations

Visual representation of the locations of the domains within the protein, as listed in the Domains and Classification table. Each row displays the domain(s) derived from a different Source, with domains color-coded according to this Source.

Scroll over a domain to view its exact coordinates and its Description.

Shared Domains

This diagram displays domains (colored squares) shared between the given protein (yellow circle) and other proteins (gray circles); the domains are color-coded according to their source, as displayed in the Domain Locations table, above.

Reset

Click on a gene or domain name to go to its specific page within SGD; drag any of the gene or domain objects around within the visualization for easier viewing; click “Reset” to automatically redraw the diagram.

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.


ecm17-A979T | ecm17-E1356K | ecm17-G1115D | ecm17-Δ | met5-Δ

View all MET5 alleles in SGD search

Sequence

Protein sequence for the given gene in S288C and other strains, when available. Use the pull-down menu under "Strain" to select the sequence for a specific strain. The displayed sequence can be downloaded in FASTA format as a .txt file. Amino acids displayed in blue represent modification sites. More detailed evidence for these modification sites is presented in the Post-translational Modifications table, located just below the protein sequence.


1 MTASDLLTLP QLLAQYSSSA PQNKVFYTTS TKNSHSSFKG LESVATDATH LLNNQDPLNT
61 IKDQLSKDIL TTVFTDETTL VKSIHHLYSL PNKLPLVITV DLNLQDYSAI PALKDLSFPI
121 LISSDLQTAI SNADSSYKIA TSSLTPVFHF LNLEKIGTST AIEQDIDFPT LEIANEETKV
181 ALSEATDSLT NFELVKGKES ITTVIVNLSP YDAEFSSVLP SNVGLIKIRV YRPWNFSKFL
241 EILPSSVTKI AVLQGVSKKS QSNEFQPFLL DFFGNFNELV SRNIEQVVLT NIGNVNDYGN
301 VINTVISNIN KKEPDNNLFL GESNEKAEEQ AEVTQLISSV KKVVNLEDAY IKVLKQLFSS
361 NLQILNQFSS ETIEPSNPEF GFGRFLKQEA QREELISLAK TSLDPSLYLS EDANKIVQLL
421 SKWLSFNGRD LDEAQLQEAN ATGLEIFQLL QSNQDSSTVL KFLKIAPTSD SFIFKSSWLI
481 GSDAWSYDLG HSGIQQVLSS RKNINVLLID SEPYDHRKQN QDRKKDVGLY AMNYYSAYVA
541 SVAVYASYTQ LLTAIIEASK YNGPSIVLAY LPYNSENDTP LEVLKETKNA VESGYWPLYR
601 FNPVYDDPST DKEAFSLDSS VIRKQLQDFL DRENKLTLLT RKDPSLSRNL KQSAGDALTR
661 KQEKRSKAAF DQLLEGLSGP PLHVYYASDG GNAANLAKRL AARASARGLK ATVLSMDDII
721 LEELPGEENV VFITSTAGQG EFPQDGKSFW EALKNDTDLD LASLNVAVFG LGDSEYWPRK
781 EDKHYFNKPS QDLFKRLELL SAKALIPLGL GDDQDADGFQ TAYSEWEPKL WEALGVSGAA
841 VDDEPKPVTN EDIKRESNFL RGTISENLKD TSSGGVTHAN EQLMKFHGIY TQDDRDIREI
901 RKSQGLEPYY MFMARARLPG GKTTPQQWLA LDHLSDTSGN GTLKLTTRAT FQIHGVLKKN
961 LKHTLRGMNA VLMDTLAAAG DVNRNVMVSA LPTNAKVHQQ IADMGKLISD HFLPKTTAYH
1021 EVWLEGPEEQ DDDPSWPSIF ENRKDGPRKK KTLVSGNALV DIEPIYGPTY LPRKFKFNIA
1081 VPPYNDVDVL SIDVGLVAIV NPETQIVEGY NVFVGGGMGT THNNKKTYPR LGSCLGFVKT
1141 EDIIPPLEGI VIVQRDHGDR KDRKHARLKY TVDDMGVEGF KQKVEEYWGK KFEPERPFEF
1201 KSNIDYFGWI KDETGLNHFT AFIENGRVED TPDLPQKTGI RKVAEYMLKT NSGHFRLTGN
1261 QHLVISNITD EHVAGIKSIL KTYKLDNTDF SGLRLSSSSC VGLPTCGLAF AESERFLPDI
1321 ITQLEDCLEE YGLRHDSIIM RMTGCPNGCS RPWLGELALV GKAPHTYNLM LGGGYLGQRL
1381 NKLYKANVKD EEIVDYIKPL FKRYALEREE GEHFGDFCIR VGIIKPTTEG KYFHEDVSED
1441 AY*

* Blue amino acids indicate modification sites. More information below.

Post-translational Modifications - S288C

Modification sites for the protein in the selected strain, based on the presence of a residue in the specific strain, as inferred from experimental evidence.

21 entries for 14 sites

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SiteModificationModifierReference
S83phosphorylated residueLeutert M, et al. (2023) PMID: 37845410
S246phosphorylated residueAlbuquerque CP, et al. (2008) PMID: 18407956
S645phosphorylated residueLanz MC, et al. (2021) PMID: 33491328
S645phosphorylated residueLeutert M, et al. (2023) PMID: 37845410
S647phosphorylated residueLeutert M, et al. (2023) PMID: 37845410
S647phosphorylated residueLanz MC, et al. (2021) PMID: 33491328
T863phosphorylated residueLanz MC, et al. (2021) PMID: 33491328
S865phosphorylated residueLanz MC, et al. (2021) PMID: 33491328
S872phosphorylated residueLeutert M, et al. (2023) PMID: 37845410
S873phosphorylated residueLanz MC, et al. (2021) PMID: 33491328
Showing 1 to 10 of 21 entries

Sequence-Based Physico-chemical Properties - S288C

Calculated protein properties, including amino acid composition, length, coding region calculations, and atomic composition.

Amino Acid Composition

Sort table using the arrow to the right of a column header to sort by that column; download all properties as a .txt file using the "Download Properties" button.

Amino AcidFrequencyPercentage
A886.10
C70.49
D976.73
E976.73
F634.37
G896.17
H281.94
I815.62
K1027.07
L16211.23
M161.11
N825.69
P664.58
Q563.88
R523.61
S1188.18
T876.03
V855.89
W151.04
Y513.54

Physical Details

Length (a.a): 1442
Molecular Weight (Da): 161180.1
Isoelectric Point (pl): 5.15
Formula: C7222H11271N1909O2225S23
Aliphatic Index: 85.58
Instability Index: 35.51

Coding Region Translation Calculations

Codon Bias: 0.26
Codon Adaptation Index: 0.25
Frequence of Optimal Codons: 0.57
Hydropathicity of Protein: -0.37
Aromaticity Score: 0.09

Extinction Coefficients at 280nm

ALL Cys residues appear as half cystines: 158865.0
NO Cys residues appear as half cystines: 158490.0

Atomic Composition

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Atom Frequency Percentage

Data not found or not available for  S288C

External Identifiers

List of external identifiers for the protein from various database sources.

15 entries for 9 sources


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External IDSource
orf19.4099CGD
1.8.1.2ExPASy
Z49637GenBank/EMBL/DDBJ
14318438GenBank/EMBL/DDBJ
1352934GenBank/EMBL/DDBJ
CAA89669.1GenBank/EMBL/DDBJ
2380LoQAtE
NM_001181795.1NCBI
DAA08922.1NCBI
853602NCBI
Showing 1 to 10 of 15 entries

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