Phenotype Help

NUP85 / YJR042W Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided.



Annotations

A phenotype is defined as an observable (e.g., apoptosis) and a qualifier (e.g., increased). There may be more than one row with the same phenotype if that phenotype was observed in separate studies or in different conditions, strains, alleles, etc.

15 entries for 11 phenotypes


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.

PhenotypeExperiment TypeMutant InformationStrain BackgroundChemicalDetailsReference
chemical compound excretion: increased
systematic mutation set

Dox titratable strains: Tet-regulated promoter that is shut off by the addition of doxycycline

repressibleS288C inositolDetails: Opi- phenotype; overproduction and excretion of inositol in the absence of inositol and choline
Salas-Santiago B and Lopes JM (2014) PMID:24558266
chromosome/plasmid maintenance: abnormal
large-scale surveyconditional
Allele: nup85-ts
S288CAssay: a-like faker (ALF) assay
Temperature: semi-permissive temperature
Details: indicative of chromosome instability (CIN); metascore weak (multiple hit)
Stirling PC, et al. (2011) PMID:21552543
chromosome/plasmid maintenance: decreased
large-scale surveyrepressibleS288C10 ug/ml doxycyclineDetails: Tet-regulated essential ORF strain collection (doxycycline represses expression)|; increased chromosome rearrangements and chromosome loss, as measured in an illegitimate mating assay
Cheng E, et al. (2012) PMID:22673806
colony sectoring: increased
large-scale surveyconditional
Allele: nup85-ts
S288CAssay: chromosome transmission fidelity (CTF) assay
Temperature: semi-permissive temperature
Details: indicative of chromosome instability (CIN); metascore weak (multiple hit)
Stirling PC, et al. (2011) PMID:21552543
haploinsufficient
heterozygous diploid, systematic mutation set null
Allele: nup85-Δ
S288COhnuki S and Ohya Y (2018) PMID:29768403
haploproficient
heterozygous diploid, competitive growth

genome-wide fitness profiling

null
Allele: nup85-Δ
S288CMedia: turbidostat growth in FPM medium
Details: Relative growth score: 0.0032
Pir P, et al. (2012) PMID:22244311
heat sensitivity: increased
heterozygous diploid, systematic mutation set

pooled screening for fitness defects at 37 deg C relative to 30 deg C

null
Allele: nup85-Δ
S288CTemperature: elevated temperature, 37 °C
Sinha H, et al. (2008) PMID:18780730
inviable
systematic mutation setnull
Allele: nup85-Δ
S288CGiaever G, et al. (2002) PMID:12140549
nuclear export: abnormal
classical geneticsconditional
Allele: nup85-rat9-1

nonsense mutation truncates protein at 404 amino acids

OtherTemperature: elevated temperature, 37 °C
Details: increased retention of poly(A)+ RNA in the nucleus
Goldstein AL, et al. (1996) PMID:8816998
nuclear export: normal
Reporter: scR1
classical geneticsconditional
Allele: nup85-ΔN
OtherTemperature: permissive temperature, 23 °C
Grosshans H, et al. (2001) PMID:11352936
Showing 1 to 10 of 15 entries

Shared Phenotypes

This diagram displays phenotype observables (purple squares) that are shared between the given gene (yellow circle) and other genes (gray circles) based on the number of phenotype observables shared (adjustable using the slider at the bottom).


Reset

Click on a gene or phenotype observable name to go to its specific page within SGD; drag any of the gene or observable objects around within the visualization for easier viewing; click “Reset” to automatically redraw the diagram; filter the genes that share observable terms with the given gene by the number of terms they share by clicking anywhere on the slider bar or dragging the tab to the desired filter number.


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