Phenotype Help

CIS3 / YJL158C Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided.


Summary
Non-essential gene; null mutation causes defects indicative of weakened cell walls, such as reduced resistance to low pH, SDS, Calcofluor White and caffeine; null mutant is also sensitive to amphotericin B but resistant to cystamine; null mutant has lower survival rate in stationary phase; in systematic studies null mutant is auxotrophic for inositol and has higher competitive fitness

Annotations

A phenotype is defined as an observable (e.g., apoptosis) and a qualifier (e.g., increased). There may be more than one row with the same phenotype if that phenotype was observed in separate studies or in different conditions, strains, alleles, etc.

26 entries for 16 phenotypes


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.

PhenotypeExperiment TypeMutant InformationStrain BackgroundChemicalDetailsReference
acid pH resistance: decreased
classical geneticsnull
Allele: cis3-Δ
SEY6210Media: low pH, pH 3.0
Mazán M, et al. (2008) PMID:18278564
adhesion: decreased
classical geneticsnull
Allele: cis3-Δ
S288CDetails: reduced in vitro adhesion to cells of enteropathogenic bacteria
Tiago FCP, et al. (2012) PMID:22580913
auxotrophy
homozygous diploid, systematic mutation setnull
Allele: cis3-Δ
S288C myo-inositolVilla-García MJ, et al. (2011) PMID:21136082
chemical compound accumulation: abnormal
systematic mutation setnull
Allele: cis3-Δ
S288C alpha-amino acidDetails: Significantly altered free amino acid profile (X^2- test p < 0.01)
Mülleder M, et al. (2016) PMID:27693354
colony appearance: abnormal
classical genetics null
Allele: cis3-Δ
OtherDetails: decrease in the degree of colony structure relative to wt in late stage (day 5) colonies of F13, a strain with a clearly-defined, highly-structured outer ring (ruffled periphery) and a smooth, unstructured center; null mutant colonies remain fully smooth at day-5
Cromie GA, et al. (2024) PMID:39637084
competitive fitness: increased
competitive growth

fitness profiling using complete deletion alleles

null
Allele: cis3-Δ
S288CMedia: minimal medium
Details: Relative fitness score: 1.014
Breslow DK, et al. (2008) PMID:18622397
competitive fitness: increased
systematic mutation setnull
Allele: cis3-Δ
S288C ethanolQian W, et al. (2012) PMID:23103169
competitive fitness: increased
systematic mutation setnull
Allele: cis3-Δ
S288CMedia: synthetic complete medium, SC
Qian W, et al. (2012) PMID:23103169
resistance to chemicals: decreased
classical geneticsnull
Allele: cis3-Δ
SEY62102 ug/ml amphotericin BMazán M, et al. (2008) PMID:18278564
resistance to chemicals: decreased
classical geneticsnull
Allele: cis3-Δ
SEY62100.0067% sodium dodecyl sulfateMazán M, et al. (2008) PMID:18278564
Showing 1 to 10 of 26 entries

Shared Phenotypes

This diagram displays phenotype observables (purple squares) that are shared between the given gene (yellow circle) and other genes (gray circles) based on the number of phenotype observables shared (adjustable using the slider at the bottom).


Reset

Click on a gene or phenotype observable name to go to its specific page within SGD; drag any of the gene or observable objects around within the visualization for easier viewing; click “Reset” to automatically redraw the diagram; filter the genes that share observable terms with the given gene by the number of terms they share by clicking anywhere on the slider bar or dragging the tab to the desired filter number.


Resources