Phenotype Help

IME2 / YJL106W Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided.


Summary
Non-essential gene; mutations increase rate of chromosome loss and reduce meiotic recombination and sporulation; overexpression causes delay in cell cycle progression through M phase and increased mitotic recombination

Annotations

A phenotype is defined as an observable (e.g., apoptosis) and a qualifier (e.g., increased). There may be more than one row with the same phenotype if that phenotype was observed in separate studies or in different conditions, strains, alleles, etc.

21 entries for 17 phenotypes


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.

PhenotypeExperiment TypeMutant InformationStrain BackgroundChemicalDetailsReference
cell cycle progression: abnormal
systematic mutation setoverexpressionS288CDetails: accumulation of cells with a 2C DNA content and an increased percentage of large-budded cells indicative of a G2 or M phase delay or arrest
Niu W, et al. (2008) PMID:18617996
chromosome/plasmid maintenance: decreased
heterozygous diploidnull
Allele: ime2-Δ
OtherDetails: >2-fold increase in the rate of chromosome loss, indicative of chromosome instability (CIN)
Strome ED, et al. (2008) PMID:18245329
chronological lifespan: increased
systematic mutation set null
Allele: ime2-Δ
S288CCampos SE, et al. (2018) PMID:29575540
colony sectoring: increased
homozygous diploidoverexpressionOtherDetails: elevated sectoring due to loss of a supernumerary chromosome fragment, an indicator of chromosome instability
Frumkin JP, et al. (2016) PMID:27530428
colony sectoring: increased
heterozygous diploid, systematic mutation setnull
Allele: ime2-Δ
S288CAssay: quantitative chromosome transmission fidelity (qCTF) assay
Details: >1.5 fold increase in mini-chromosome loss relative to wt, indicative of chromosome instability (CIN)
Zhu J, et al. (2015) PMID:25823586
competitive fitness: decreased
competitive growth

fitness profiling using complete deletion alleles

null
Allele: ime2-Δ
S288CMedia: minimal medium
Details: Relative fitness score: 0.987
Breslow DK, et al. (2008) PMID:18622397
competitive fitness: decreased
competitive growth overexpressionS288CDetails: decreased abundance within the barFLEX overexpression collection arrayed after 20 generations of pooled growth
Douglas AC, et al. (2012) PMID:23050238
haploproficient
heterozygous diploid, competitive growthnull
Allele: ime2-Δ
S288CTreatment: conditions that mimic Phase II of a batch fermentation, equivalent to ~22 hours after inoculation of a batch fermentation
Novo M, et al. (2013) PMID:24040173
invasive growth: increased
homozygous diploid, systematic mutation setoverexpressionSigma1278bMedia: nitrogen-sufficient medium
Details: agar invasion score: 0.99; enhanced pseudohyphal growth, based on elevated invasiveness
Shively CA, et al. (2013) PMID:23410832
liquid culture appearance: abnormal
systematic mutation set null
Allele: ime2-Δ
S288CDetails: Creeping phenotype: When cells of opposite mating type are mixed in liquid media, they 'creep' up the culture vessel sides
Arras SDM, et al. (2022) PMID:35298616
Showing 1 to 10 of 21 entries

Shared Phenotypes

This diagram displays phenotype observables (purple squares) that are shared between the given gene (yellow circle) and other genes (gray circles) based on the number of phenotype observables shared (adjustable using the slider at the bottom).


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Click on a gene or phenotype observable name to go to its specific page within SGD; drag any of the gene or observable objects around within the visualization for easier viewing; click “Reset” to automatically redraw the diagram; filter the genes that share observable terms with the given gene by the number of terms they share by clicking anywhere on the slider bar or dragging the tab to the desired filter number.


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