Protein Help

ARP4 / YJL081C Protein

Protein abundance data, domains, shared domains with other proteins, protein sequence retrieval for various strains, sequence-based physico-chemical properties, protein modification sites, and external identifiers for the protein.


Aliases
ACT3 2
Feature Type
ORF , Verified

AlphaFold Protein Structure

AlphaFold, developed by DeepMind, is an AI program that accurately predicts protein structures from amino acid sequences, enabling visualization of protein conformations. The predicted structures can be accessed through the Protein Data Bank (PDB) and AlphaFold Protein Structure Database.


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Model Confidence

Very high
Confident
Low
Very low

Experimental Data

Contains experimentally-derived protein half-life data obtained using stable isotope labeling by amino acids (SILAC) coupled with mass spectrometry. This section also contains protein abundance data for both untreated and treated cells obtained from over 20 studies. These data have been normalized and converted to a common unit of molecules per cell.


Protein Half Life

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ExperimentResultReference
half-life7.1 hrChristiano R, et al. (2014)

Protein Abundance

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Abundance (molecules/cell)MediaTreatmentTreatment timeFold ChangeVisualizationStrain backgroundOriginal ReferenceReference
4235SDuntreatedquantitative mass spectrometry evidenceS288Cde Godoy LM, et al. (2008)Ho B, et al. (2018)
3474SDuntreatedwide-field microscopy evidenceS288CDénervaud N, et al. (2013)Ho B, et al. (2018)
1070YEPDuntreatedqualitative western immunoblotting evidenceS288CGhaemmaghami S, et al. (2003)Ho B, et al. (2018)
1164YEPDuntreatedquantitative mass spectrometry evidenceS288CKulak NA, et al. (2014)Ho B, et al. (2018)
4653YEPDuntreatedquantitative mass spectrometry evidenceS288CLee MV, et al. (2011)Ho B, et al. (2018)
Showing 1 to 5 of 12 entries

Domains and Classification - S288C

Collection of computationally identified domains and motifs, as determined by InterProScan analysis; includes protein coordinates for the domain, a domain Description, a Source and corresponding accession ID, and the number of S. cerevisiae genes that share the same domain.


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Gene Protein Coordinates Accession ID Description Source No. of Genes with Domain

Domain Locations

Visual representation of the locations of the domains within the protein, as listed in the Domains and Classification table. Each row displays the domain(s) derived from a different Source, with domains color-coded according to this Source.

Scroll over a domain to view its exact coordinates and its Description.

Shared Domains

This diagram displays domains (colored squares) shared between the given protein (yellow circle) and other proteins (gray circles); the domains are color-coded according to their source, as displayed in the Domain Locations table, above.

Reset

Click on a gene or domain name to go to its specific page within SGD; drag any of the gene or domain objects around within the visualization for easier viewing; click “Reset” to automatically redraw the diagram.

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.


arp4-1 | arp4-12 | arp4-2 | arp4-26 | arp4-3 | arp4-5 | arp4-6 | ... Show all

View all ARP4 alleles in SGD search

Sequence

Protein sequence for the given gene in S288C and other strains, when available. Use the pull-down menu under "Strain" to select the sequence for a specific strain. The displayed sequence can be downloaded in FASTA format as a .txt file. Amino acids displayed in blue represent modification sites. More detailed evidence for these modification sites is presented in the Post-translational Modifications table, located just below the protein sequence.


1 MSNAALQVYG GDEVSAVVID PGSYTTNIGY SGSDFPQSIL PSVYGKYTAD EGNKKIFSEQ
61 SIGIPRKDYE LKPIIENGLV IDWDTAQEQW QWALQNELYL NSNSGIPALL TEPVWNSTEN
121 RKKSLEVLLE GMQFEACYLA PTSTCVSFAA GRPNCLVVDI GHDTCSVSPI VDGMTLSKST
181 RRNFIAGKFI NHLIKKALEP KEIIPLFAIK QRKPEFIKKT FDYEVDKSLY DYANNRGFFQ
241 ECKETLCHIC PTKTLEETKT ELSSTAKRSI ESPWNEEIVF DNETRYGFAE ELFLPKEDDI
301 PANWPRSNSG VVKTWRNDYV PLKRTKPSGV NKSDKKVTPT EEKEQEAVSK STSPAANSAD
361 TPNETGKRPL EEEKPPKENN ELIGLADLVY SSIMSSDVDL RATLAHNVVL TGGTSSIPGL
421 SDRLMTELNK ILPSLKFRIL TTGHTIERQY QSWLGGSILT SLGTFHQLWV GKKEYEEVGV
481 ERLLNDRFR*

* Blue amino acids indicate modification sites. More information below.

Post-translational Modifications - S288C

Modification sites for the protein in the selected strain, based on the presence of a residue in the specific strain, as inferred from experimental evidence.

28 entries for 9 sites

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SiteModificationModifierReference
S309phosphorylated residueLanz MC, et al. (2021) PMID: 33491328
K323monoacetylated residueHenriksen P, et al. (2012) PMID: 22865919
T338phosphorylated residueAlbuquerque CP, et al. (2008) PMID: 18407956
T338phosphorylated residueLanz MC, et al. (2021) PMID: 33491328
T338phosphorylated residueSoulard A, et al. (2010) PMID: 20702584
T338phosphorylated residueLeutert M, et al. (2023) PMID: 37845410
T338phosphorylated residueMacGilvray ME, et al. (2020) PMID: 32597660
T340phosphorylated residueHolt LJ, et al. (2009) PMID: 19779198
S349phosphorylated residueLanz MC, et al. (2021) PMID: 33491328
S349phosphorylated residueLeutert M, et al. (2023) PMID: 37845410
Showing 1 to 10 of 28 entries

Sequence-Based Physico-chemical Properties - S288C

Calculated protein properties, including amino acid composition, length, coding region calculations, and atomic composition.

Amino Acid Composition

Sort table using the arrow to the right of a column header to sort by that column; download all properties as a .txt file using the "Download Properties" button.

Amino AcidFrequencyPercentage
A255.11
C71.43
D244.91
E449.00
F173.48
G306.13
H61.23
I306.13
K377.57
L449.00
M51.02
N275.52
P285.73
Q142.86
R204.09
S438.79
T357.16
V285.73
W91.84
Y163.27

Physical Details

Length (a.a): 489
Molecular Weight (Da): 54821.3
Isoelectric Point (pl): 5.12
Formula: C2442H3823N648O761S12
Aliphatic Index: 77.5
Instability Index: 45.85

Coding Region Translation Calculations

Codon Bias: 0.05
Codon Adaptation Index: 0.13
Frequence of Optimal Codons: 0.46
Hydropathicity of Protein: -0.49
Aromaticity Score: 0.09

Extinction Coefficients at 280nm

ALL Cys residues appear as half cystines: 73715.0
NO Cys residues appear as half cystines: 73340.0

Atomic Composition

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Atom Frequency Percentage

Data not found or not available for  S288C

External Identifiers

List of external identifiers for the protein from various database sources.

35 entries for 11 sources


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External IDSource
AN7441AspGD
orf19.5623CGD
2361DIP
X83502GenBank/EMBL/DDBJ
Z49356GenBank/EMBL/DDBJ
X75317GenBank/EMBL/DDBJ
332138221GenBank/EMBL/DDBJ
6322380GenBank/EMBL/DDBJ
728794GenBank/EMBL/DDBJ
332138219GenBank/EMBL/DDBJ
Showing 1 to 10 of 35 entries

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